An integrated system of four multiplex qPCR assays for the precise and sensitive identification of animal species in food and feed

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880004" target="_blank" >RIV/62157124:16270/22:43880004 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.foodcont.2021.108781" target="_blank" >https://doi.org/10.1016/j.foodcont.2021.108781</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodcont.2021.108781" target="_blank" >10.1016/j.foodcont.2021.108781</a>

Result language
angličtina
Original language name
An integrated system of four multiplex qPCR assays for the precise and sensitive identification of animal species in food and feed
Original language description
A system of four multiplexes has been developed and optimized to detect up to 18 animal species simultaneously. The system consists of one quadruplex making it possible to detect the most common species - cattle, pigs, and chicken - and to reveal potential inhibitors. An additional 15 meat species can be proven using another three pentaplexes. The uniqueness of the system lies not only in the multiplexing of many different samples, but also in its high sensitivity making it possible to detect 10 pg/microl of DNA or even less. Targeting specific sequences in genomic DNA instead of mitochondrial DNA represents another exclusivity of the system and further increases its specificity. The application of the system on real samples of pet foods obtained from retail showed non-declared species in up to 60% of samples. This demonstrates the great need for a precise and sensitive system for the control of food/feed adulteration and fraud.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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