Lactoferrin content determined in bovine milk by HPLC and mid-infrared spectrometry - relation to udder health and potential for detection of milk adulteration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F25%3A43882815" target="_blank" >RIV/62157124:16270/25:43882815 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12220/25:43910223 RIV/26722861:_____/25:N0000033

Result on the web

<a href="https://cjas.agriculturejournals.cz/pdfs/cjs/2025/11/04.pdf" target="_blank" >https://cjas.agriculturejournals.cz/pdfs/cjs/2025/11/04.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/114/2025-CJAS" target="_blank" >10.17221/114/2025-CJAS</a>

Result language

angličtina

Original language name

Lactoferrin content determined in bovine milk by HPLC and mid-infrared spectrometry - relation to udder health and potential for detection of milk adulteration

Original language description

Lactoferrin (LF) is a multifunctional glycoprotein linked to udder health in dairy cows. This study aimed to develop a calibration model for LF quantification using mid-infrared spectrometry (MIR-FT), with ion-pair reversedphase high-performance liquid chromatography (HPLC) as the reference method. Two sets of individual milk samples (A: n = 120; B: n = 91) were collected from five dairy farms in the Czech Republic. Set A included a higher proportion of samples with somatic cell count (SCC) above 300 000 cells/ml to ensure broad LF variability. After merging both sets and removing six outliers, a final calibration set C (n = 205) was created. The developed model achieved a crossvalidated coefficient of determination of 0.588 7 and a standard error of cross-validation of 67.33 mg/l. Correlation analysis showed that several milk parameters correlated with LF determined by MIR-FT in patterns typical of mastitis (SCC: r = 0.450; lactose: r = –0.364; free fatty acids: r = 0.621; electrical conductivity: r = 0.442), indicating potential for MIR-FT in evaluating mammary gland health. The feasibility of using LF as an indicator of milk adulteration by artificial SCC reduction through centrifugation was also assessed. Two sample sets (n = 20 and n = 68) were analysed, each containing normal bulk tank milk and bulk tank milk supplemented with abnormal milk. Centrifugation caused minimal changes in LF determined by both HPLC and MIR-FT (maximum 3.27%) while SCC decreased by nearly 50%, suggesting that LF may serve as a marker for detecting artificial SCC reduction. However, practical application of MIR-FT for accurate LF determination is limited by the achieved validation parameters and the high expanded uncertainty (114.7 mg/l). The method is therefore more suitable for monitoring relative LF changes in milk than for determining exact LF content.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

1805-9309

Volume of the periodical

70

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

467-479

UT code for WoS article

001628928700002

EID of the result in the Scopus database

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