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Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018307" target="_blank" >RIV/62690094:18470/21:50018307 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.nature.com/articles/s41598-021-89943-5" target="_blank" >https://www.nature.com/articles/s41598-021-89943-5</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41598-021-89943-5" target="_blank" >10.1038/s41598-021-89943-5</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

  • Original language description

    This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC(60) and monoglucosamineC(60), which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel-Hirsch reaction enables the chemical modification of the fullerene scaffold with the desired bioactive fragments such as d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The ESI-MS and C-13-NMR analyses of JK39 confirmed its high T-h symmetry, while X-ray photoelectron spectroscopy revealed the presence of nitrogen and oxygen-containing C-O or C-N bonds. The efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line DU145 expressing the GFP protein. The HexakisaminoC(60) fullerene was an efficient siRNA transfection agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly, the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta potential and the formation of an extremely stable complex with siRNA.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    21001 - Nano-materials (production and properties)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scientific reports

  • ISSN

    2045-2322

  • e-ISSN

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    10

  • Pages from-to

    "Article Number:10565"

  • UT code for WoS article

    000658822200002

  • EID of the result in the Scopus database

    2-s2.0-85106296468