Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018307" target="_blank" >RIV/62690094:18470/21:50018307 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-021-89943-5" target="_blank" >https://www.nature.com/articles/s41598-021-89943-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-021-89943-5" target="_blank" >10.1038/s41598-021-89943-5</a>
Alternative languages
Result language
angličtina
Original language name
Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
Original language description
This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC(60) and monoglucosamineC(60), which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel-Hirsch reaction enables the chemical modification of the fullerene scaffold with the desired bioactive fragments such as d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The ESI-MS and C-13-NMR analyses of JK39 confirmed its high T-h symmetry, while X-ray photoelectron spectroscopy revealed the presence of nitrogen and oxygen-containing C-O or C-N bonds. The efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line DU145 expressing the GFP protein. The HexakisaminoC(60) fullerene was an efficient siRNA transfection agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly, the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta potential and the formation of an extremely stable complex with siRNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
"Article Number:10565"
UT code for WoS article
000658822200002
EID of the result in the Scopus database
2-s2.0-85106296468