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Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00329247" target="_blank" >RIV/67985823:_____/09:00329247 - isvavai.cz</a>

  • Alternative codes found

    RIV/68407700:21460/09:00156738

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

  • Original language description

    A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithmto compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USBlight microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    JC - Computer hardware and software

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Stereology and Image Analysis

  • ISBN

    978-88-7488-310-3

  • ISSN

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

  • Publisher name

    Esculapio

  • Place of publication

    Bologna

  • Event location

    Milano

  • Event date

    Jun 22, 2009

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article