Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00329247" target="_blank" >RIV/67985823:_____/09:00329247 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/09:00156738
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration
Original language description
A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithmto compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USBlight microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
JC - Computer hardware and software
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Stereology and Image Analysis
ISBN
978-88-7488-310-3
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
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Publisher name
Esculapio
Place of publication
Bologna
Event location
Milano
Event date
Jun 22, 2009
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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