Valve interstitial cell culture: Production of mature type I collagen and precise detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00477592" target="_blank" >RIV/67985823:_____/17:00477592 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11510/17:10359550 RIV/00023001:_____/17:00075981
Result on the web
<a href="http://dx.doi.org/10.1002/jemt.22886" target="_blank" >http://dx.doi.org/10.1002/jemt.22886</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jemt.22886" target="_blank" >10.1002/jemt.22886</a>
Alternative languages
Result language
angličtina
Original language name
Valve interstitial cell culture: Production of mature type I collagen and precise detection
Original language description
Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions, with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures. Visualization of only extracellular collagen returned a higher correlation coefficient when comparing the immunolabeling and second harmonic generation microscopy images in the culture with ascorbic acid. Lastly, it was proved that the hydroxyproline strongly contributes to the second-order susceptibility tensor of collagen molecules, and therefore the second harmonic generation signal is impaired in the culture without ascorbic acid.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy Research and Technique
ISSN
1059-910X
e-ISSN
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Volume of the periodical
80
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
936-942
UT code for WoS article
000406242500015
EID of the result in the Scopus database
2-s2.0-85018361386