cytotoxicity of polyspermine-ribonuclease A and polyspermine-dimeric ribonuclease A

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F07%3A00090417" target="_blank" >RIV/67985904:_____/07:00090417 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/07:00008922 RIV/00023736:_____/07:00007198

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

cytotoxicity of polyspermine-ribonuclease A and polyspermine-dimeric ribonuclease A

Original language description

Polyspermine-ribonuclease A (PS-RNase A) and polyspermine-dimeric ribonuclease A (PS-dimeric RNase A) were prepared by cross-linking ribonuclease A or its covalently linked dimer to polyspermine (PS) using dimethyl suberimidate. The two RNase A derivatives were tested for a possible antitumor action. The in vitro and in vivo cytotoxic activity of PS-RNase A, although strong, is not higher than that known for free polyspermine. PS-dimeric RNase A, which was characterized by mass spectroscopy, titration of free amine groups, and enzymatic assays, proved instead to be a definitely more efficient antitumor agent, both in vitro and in vivo. This result could tentatively be explained in view of the importance of positive charges for ribonuclease activity, considering the higher basicity of PS-dimeric RNase A compared to that of PS-(monomeric)RNase A. It must be also taken into account that the dimeric RNase A moiety of PS-dimeric RNase A could evade the cytoplasmic ribonuclease inhibitor, wh

Czech name

Cytotoxicita polyspermine-ribonukleázy A a polyspermine-dimericke ribonukleázy A

Czech description

Polyspermine-ribonukleáza A a polyspermine - dimerická ribonukleáza A byly připraveny metodou cross-linking ribonukleázy A nebo jejím kovalentně navázaným dimerem k polysperminu pomocí dimethyl suberemidátu. Cytotoxická aktivita vyjádřena protinádorovýmefektem in vitro i in vivo byla u prvního derivátu ribonukleázy A silná, ale u dimerické ribonukleázy A byla protinádorově silnější, což je vyvoláno vyšší bosicitou tohoto komplexu.Výsledky této práce demonstrují důležitost savčích ribonukleáz A, vláštěpankreatické A RNázy pro potenciální terapeutické využití.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioconjugate Chemistry

ISSN

1043-1802

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

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Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

1946-1955

UT code for WoS article

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EID of the result in the Scopus database

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