Chronic Hepatitis C Virus Infection Modulates the Transcriptional Profiles of CD4(+) T Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00541961" target="_blank" >RIV/67985904:_____/21:00541961 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/21:10427910 RIV/61383082:_____/21:00001065 RIV/00216208:11110/21:10427910 RIV/00064211:_____/21:W0000024 RIV/00179906:_____/21:10427910
Result on the web
<a href="https://www.hindawi.com/journals/cjidmm/2021/6689834/" target="_blank" >https://www.hindawi.com/journals/cjidmm/2021/6689834/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2021/6689834" target="_blank" >10.1155/2021/6689834</a>
Alternative languages
Result language
angličtina
Original language name
Chronic Hepatitis C Virus Infection Modulates the Transcriptional Profiles of CD4(+) T Cells
Original language description
Background. Chronic hepatitis C (CHC) is associated with altered cell-mediated immune response. Objective. The aim of the study was to characterize functional alterations in CD4(+) T cell subsets and myeloid-derived suppressor cells (MDSCs) during chronic hepatitis C virus (HCV) infection. Methodology. The expression levels of the lineage-defining transcriptional factors (TFs) T-bet, Gata3, Roryt, and Foxp3 in circulating CD4(+) T cells and percentages of MDSCs in peripheral blood were evaluated in 33 patients with CHC, 31 persons, who had spontaneously cleared the HCV infection, and 30 healthy subjects. Analysis. The CD4(+) T cells TFs T-bet (T-box expressed in T cells), Foxp3 (Forkhead box P3 transcription factor), Gata3 (Gata-binding protein 3), and Roryt (retinoic-acid-related orphan receptor gamma) and activation of CD8(+) T cells, as well as percentages of MDSCs, were measured by multicolor flow cytometry after intracellular and surface staining of peripheral blood mononuclear cells with fluorescent monoclonal antibodies. Result. The patients with CHC had significantly lower percentages of CD4(+) T cells expressing Roryt and Gata3 and higher percentages of Foxp3-expressing CD4(+) T cells than healthy controls and persons who spontaneously cleared HCV infection. The ratios of T-bet(+)/Gata3(+) and Foxp3(+)/Ror gamma t(+) CD4(+) T cells were the highest in the patients with CHC. In the patients with CHC, the percentages of Gata3(+) and Ror gamma t CD4(+) T cells and the percentages of T-bet(+) CD4(+) T cells and CD38(+)/ HLA-DR+ CD8(+) T cells demonstrated significant positive correlations. In addition, the percentage of CD38(+)/HLA-DR+ CD8(+) T cells correlated negatively with the percentage of MDSCs. Conclusion. Chronic HCV infection is associated with downregulation of TFs Gata3 and Roryt polarizing CD4+ T cells into Th2 and Th17 phenotypes together with upregulation of Foxp3 responsible for induction of regulatory T cells suppressing immune response.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/NT14072" target="_blank" >NT14072: Predictive immunological markers in patients with hepatitis C viral infection</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Canadian Journal of Infectious Diseases & Medical Microbiology
ISSN
1712-9532
e-ISSN
1918-1493
Volume of the periodical
2021
Issue of the periodical within the volume
MAR 12
Country of publishing house
CA - CANADA
Number of pages
6
Pages from-to
6689834
UT code for WoS article
000631966800001
EID of the result in the Scopus database
2-s2.0-85102987482