Micro-morphological Characterization of In-Vivo Diatoms Using ESEM

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F17%3A00481040" target="_blank" >RIV/67985939:_____/17:00481040 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/17:00481040

Result on the web

<a href="http://dx.doi.org/10.1017/S1431927617007395" target="_blank" >http://dx.doi.org/10.1017/S1431927617007395</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927617007395" target="_blank" >10.1017/S1431927617007395</a>

Result language

angličtina

Original language name

Micro-morphological Characterization of In-Vivo Diatoms Using ESEM

Original language description

Diatoms (Bacillariophyta) are one of the most distinctive and successful groups of unicellula photosynthetic algae. They are ecologically widespread inhabiting mainly fresh, brackish and marine waters, often dominant in given microhabitats and contribute about one fourth to the world’s productivity (in terms of carbon fixation). Diatoms are also the most species-rich algal group represented by 50 – 200 thousand species. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule which is composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating. This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of: 1) whole intact diatom cells, not only empty diatom frustules, 2) extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes), 3) whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating. Observation of these aquatic organisms in their native wet state is not yet common even if it allows study of diatoms adhered directly on the host plant. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method for the ESEM (LTM), ideally in a combination with advanced low noise detectors with very high detection efficiency.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

S1

Country of publishing house

US - UNITED STATES

Number of pages

2

Pages from-to

1346-1347

UT code for WoS article

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EID of the result in the Scopus database

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