The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00504760" target="_blank" >RIV/68081731:_____/18:00504760 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/18:00504760 RIV/61989592:15310/19:73596008
Result on the web
<a href="http://hdl.handle.net/11104/0296319" target="_blank" >http://hdl.handle.net/11104/0296319</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/0269249X.2018.1545703" target="_blank" >10.1080/0269249X.2018.1545703</a>

Result language
angličtina
Original language name
The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo
Original language description
The Low Temperature Method for sample stabilization in environmental scanning electron microscopy (LTM for ESEM) is a promising tool for the observation of diatom assemblages in vivo. Use of the environmental scanning electron microscope, in comparison to the conventional scanning electron microscope, enables study of fresh material without any chemical pretreatment and conductive coating. The newly developed Low Temperature Method, introduced in this paper, offers higher resolution and better resistance of wet samples to radiation damage. We used natural epiphytic algal assemblages to image 3D structure of: i) biofilm/periphyton and its physical complexity, ii) diatoms with their extracellular mucilaginous secretions enabling cells to attach to the substrate, iii) diatom colony formation, and iv) intact diatom cells in contrast to separated empty valves observed in the conventional scanning electron microscope. This study demonstrates the potential of this new method for environmental scanning electron microscopy in diatom biology and ecology in comparison with other imaging methods.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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