In vivo study of diatom assemblages using low temperature method for ESEM

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F17%3A00481582" target="_blank" >RIV/67985939:_____/17:00481582 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/17:00481582

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In vivo study of diatom assemblages using low temperature method for ESEM

Original language description

Diatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

13th Multinational Congress on Microscopy: Book of Abstracts

ISBN

978-953-7941-19-2

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

64-65

Publisher name

Ruder Bošković Institute, Croatian Microscopy Society

Place of publication

Zagreb

Event location

Rovinj

Event date

Sep 24, 2017

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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