How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles?

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00428114" target="_blank" >RIV/68081707:_____/14:00428114 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/1471-2156-15-2" target="_blank" >http://dx.doi.org/10.1186/1471-2156-15-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1471-2156-15-2" target="_blank" >10.1186/1471-2156-15-2</a>

Result language
angličtina
Original language name
How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles?
Original language description
Background: DNA methylation plays a key role in development, contributes to genome stability, and may also respond to external factors supporting adaptation and evolution. To connect different types of stimuli with particular biological processes, identifying genome regions with altered 5-methylcytosine distribution at a genome-wide scale is important. Many researchers are using the simple, reliable, and relatively inexpensive Methylation Sensitive Amplified Polymorphism (MSAP) method that is particularly useful in studies of epigenetic variation. However, electrophoretic patterns produced by the method are rather difficult to interpret, particularly when MspI and HpaII isoschizomers are used because these enzymes are methylation-sensitive, and any C within the CCGG recognition motif can be methylated in plant DNA.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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