Fast enzyme-linked electrochemical sensing of DNA hybridization at pencil graphite electrodes. Application to detect gene deletion in a human cell culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00524530" target="_blank" >RIV/68081707:_____/20:00524530 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/20:00118310

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S157266572030134X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S157266572030134X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2020.113951" target="_blank" >10.1016/j.jelechem.2020.113951</a>

Result language

angličtina

Original language name

Fast enzyme-linked electrochemical sensing of DNA hybridization at pencil graphite electrodes. Application to detect gene deletion in a human cell culture

Original language description

In this paper we present a rapid electrochemical enzyme-linked DNA hybridization assay using disposable pencil graphite electrodes (PeGE) to detect target DNA (tDNA) sequences in DNA fragments amplified by polymerase chain reaction. The procedure consists of several short (1-2 min) incubation steps, including adsorption of the tDNA at unpretreated PeGE from denaturing medium, surface blocking with milk proteins, hybridization with a biotinylated oligonucleotide probe and binding of streptavidin-alkaline phosphatase conjugate to the biotin tags. Then the PeGE is transferred into background electrolyte solution containing 1-naphthyl phosphate, which is enzymatically dephosphorylated to give electrochemically oxidizable indicator 1-naphthol. The assay, which can be performed within 7-8 min, offers a perfect discrimination between specific and nonspecific DNA amplicons and easy detection of about similar to 40 femtomoles of tDNA in large excesses of non-complementary DNA. An application on the detection of p53 gene deletion in a human cell culture, featuring a real biologicalmodel, is presented. The designed setup has a potential to be applied as one of simple, fast, robust ultralow cost do-it-yourself instruments recently introduced as diagnostic tools for third world countries. (c) 2020 Elsevier B.V. All rights reserved.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

<a href="/en/project/GAP206%2F11%2F1638" target="_blank" >GAP206/11/1638: Novel electrochemical sensors and sensing techniques for the analysis of nucleic acids structure and interactions</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Volume of the periodical

862

Issue of the periodical within the volume

APR 1 2020

Country of publishing house

CH - SWITZERLAND

Number of pages

7

Pages from-to

113951

UT code for WoS article

000525903900004

EID of the result in the Scopus database

2-s2.0-85080093599