Tools for visualization of phosphoinositides in the cell nucleus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F16%3A00472618" target="_blank" >RIV/68378050:_____/16:00472618 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/16:10336679

Result on the web

<a href="http://dx.doi.org/10.1007/s00418-016-1409-8" target="_blank" >http://dx.doi.org/10.1007/s00418-016-1409-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00418-016-1409-8" target="_blank" >10.1007/s00418-016-1409-8</a>

Result language

angličtina

Original language name

Tools for visualization of phosphoinositides in the cell nucleus

Original language description

Phosphoinositides (PIs) are glycerol-based phospholipids containing hydrophilic inositol ring. The inositol ring is mono-, bis-, or tris-phosphorylated yielding seven PIs members. Ample evidence shows that PIs localize both to the cytoplasm and to the nucleus. However, tools for direct visualization of nuclear PIs are limited and many studies thus employ indirect approaches, such as staining of their metabolic enzymes. Since localization and mobility of PIs differ from their metabolic enzymes, these approaches may result in incomplete data. In this paper, we tested commercially available PIs antibodies by light microscopy on fixed cells, tested their specificity using protein-lipid overlay assay and blocking assay, and compared their staining patterns. Additionally, we prepared recombinant PIs-binding domains and tested them on both fixed and live cells by light microscopy. The results provide a useful overview of usability of the tools tested and stress that the selection of adequate tools is critical. Knowing the localization of individual PIs in various functional compartments should enable us to better understand the roles of PIs in the cell nucleus.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

—

Volume of the periodical

145

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

485-496

UT code for WoS article

000372608900012

EID of the result in the Scopus database

—