Polymer cancerostatics targeted with an antibody fragment bound via a coiled coil motif: in vivo therapeutic efficacy against murine BCL1 leukemia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F18%3A00484884" target="_blank" >RIV/68378050:_____/18:00484884 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00484884 RIV/61389013:_____/18:00484884
Result on the web
<a href="http://dx.doi.org/10.1002/mabi.201700173" target="_blank" >http://dx.doi.org/10.1002/mabi.201700173</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mabi.201700173" target="_blank" >10.1002/mabi.201700173</a>
Alternative languages
Result language
angličtina
Original language name
Polymer cancerostatics targeted with an antibody fragment bound via a coiled coil motif: in vivo therapeutic efficacy against murine BCL1 leukemia
Original language description
A BCL1 leukemia-cell-targeted polymer–drug conjugate with a narrow molecular weight distribution consisting of an N-(2-hydroxypropyl)methacrylamide copolymer carrier and the anticancer drug pirarubicin is prepared by controlled radical copolymerization followed by metal-free click chemistry. A targeting recombinant single chain antibody fragment (scFv) derived from a B1 monoclonal antibody is attached noncovalently to the polymer carrier via a coiled coil interaction between two complementary peptides. Two pairs of coiled coil forming peptides (abbreviated KEK/EKE and KSK/ESE) are used as linkers between the polymer-pirarubicin conjugate and the targeting protein. The targeted polymer conjugate with the coiled coil linker KSK/ESE exhibits 4× better cell binding activity and 2× higher cytotoxicity in vitro compared with the other conjugate. Treatment of mice with established BCL1 leukemia using the scFv-targeted polymer conjugate leads to a markedly prolonged survival time of the experimental animals compared with the treatment using the free drug and the nontargeted polymer-pirarubicin conjugate.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecular Bioscience
ISSN
1616-5187
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
1-11
UT code for WoS article
000427446200008
EID of the result in the Scopus database
2-s2.0-85040669920