Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00521819" target="_blank" >RIV/68378050:_____/19:00521819 - isvavai.cz</a>
Result on the web
<a href="https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008261" target="_blank" >https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008261</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pgen.1008261" target="_blank" >10.1371/journal.pgen.1008261</a>
Alternative languages
Result language
angličtina
Original language name
Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes
Original language description
Germline genome defense evolves to recognize and suppress retrotransposons. One of defensive mechanisms is the PIWI-associated RNA (piRNA) pathway, which employs small RNAs for sequence-specific repression. The loss of the piRNA pathway in mice causes male sterility while females remain fertile. Unlike spermatogenic cells, mouse oocytes posses also RNA interference (RNAi), another small RNA pathway capable of retrotransposon suppression. To examine whether RNAi compensates the loss of the piRNA pathway, we produced a new RNAi pathway mutant DicerSOM and crossed it with a catalytically-dead mutant of Mili, an essential piRNA gene. Normal follicular and oocyte development in double mutants showed that RNAi does not suppress a strong ovarian piRNA knock-out phenotype. However, we observed redundant and non-redundant targeting of specific retrotransposon families illustrating stochasticity of recognition and targeting of invading retrotransposons. Intracisternal A Particle retrotransposon was mainly targeted by the piRNA pathway, MaLR and RLTR10 retrotransposons were targeted mainly by RNAi. Double mutants showed accumulations of LINE-1 retrotransposon transcripts. However, we did not find strong evidence for transcriptional activation and mobilization of retrotransposition competent LINE-1 elements suggesting that while both defense pathways are simultaneously expendable for ovarian oocyte development, yet another transcriptional silencing mechanism prevents mobilization of LINE-1 elements.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS Genetics
ISSN
1553-7404
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
e1008261
UT code for WoS article
000512336600004
EID of the result in the Scopus database
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