Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F18%3A00322095" target="_blank" >RIV/68407700:21220/18:00322095 - isvavai.cz</a>
Result on the web
<a href="http://journals.tubitak.gov.tr/biology/issues/biy-18-42-2/biy-42-2-10-1711-26.pdf" target="_blank" >http://journals.tubitak.gov.tr/biology/issues/biy-18-42-2/biy-42-2-10-1711-26.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3906/biy-1711-26" target="_blank" >10.3906/biy-1711-26</a>
Alternative languages
Result language
angličtina
Original language name
Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells
Original language description
In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2-2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hAD-MSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er') with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/GA16-14758S" target="_blank" >GA16-14758S: Influence of surface nanotopography on bioactive properties of low modulus titanium alloy.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
TURKISH JOURNAL OF BIOLOGY
ISSN
1300-0152
e-ISSN
1300-0152
Volume of the periodical
42
Issue of the periodical within the volume
2
Country of publishing house
TR - TURKEY
Number of pages
9
Pages from-to
195-203
UT code for WoS article
000433481900010
EID of the result in the Scopus database
2-s2.0-85046034278