Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F20%3A00013464" target="_blank" >RIV/75010330:_____/20:00013464 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41330/20:88249 RIV/61989592:15110/20:73606867
Result on the web
<a href="http://www.biomed.cas.cz/physiolres/pdf/2020/69_S661.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/2020/69_S661.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934615" target="_blank" >10.33549/physiolres.934615</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens
Original language description
Growing worldwide efforts to replace (reduce) animal testing and to improve alternative in vitro tests which may be more efficient in terms of both time, cost and scientific validity include also genotoxicity/mutagenicity endpoints. The aim of the review article was to summarize currently available in vitro testing approaches in this field, their regulatory acceptance and recommended combinations for classification of chemicals. A study using the combination of Comet Assay performed on two cell lines and the Chromosomal Aberration test on human peripheral lymphocytes was performed with the aim to predict the genotoxic potential of selected paraben esters, serving as a model chemical group. Parabens are widely used in consumer products as preservatives and have been reported to exhibit inconclusive results in numerous genotoxicity studies. The Comet Assay identified Ethylparaben and Benzylparaben as potentially genotoxic. The Chromosomal Aberration test revealed weak genotoxic potential in case of Ethylparaben and positive genotoxicity in case of Butylparaben, Propylparaben and Isopropylparaben. The main reasons for variability seem to be limited water solubility of parabens, determining their bioavailability at the cellular level, and absence of metabolic activation in the Comet Assay. The results confirmed that the Comet Assay should serve as a screening test and should not be used as a stand-alone method for classification of genotoxicity. The weight of evidence approach in risk assessment should be supported with data generated with the use of human relevant in vitro methods based on cells / tissues of human origin.
Czech name
—
Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology
Result continuities
Project
<a href="/en/project/EF16_019%2F000860" target="_blank" >EF16_019/000860: International competitiveness of NIPH in research, development and education in alternative toxicological methods.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
1802-9973
Volume of the periodical
69
Issue of the periodical within the volume
Suppl. 4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
19
Pages from-to
„S661“-„S679“
UT code for WoS article
000621838200012
EID of the result in the Scopus database
2-s2.0-85102229594