Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00379059" target="_blank" >RIV/86652036:_____/12:00379059 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.clinbiochem.2012.02.009" target="_blank" >http://dx.doi.org/10.1016/j.clinbiochem.2012.02.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.clinbiochem.2012.02.009" target="_blank" >10.1016/j.clinbiochem.2012.02.009</a>

Result language

angličtina

Original language name

Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

Original language description

Objectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F08%2F0811" target="_blank" >GA204/08/0811: Mechanism of ROS generation and mitochondrial permeabilization</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical Biochemistry

ISSN

0009-9120

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

7-8

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

575-581

UT code for WoS article

000303553300013

EID of the result in the Scopus database

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