SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00398939" target="_blank" >RIV/86652036:_____/13:00398939 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/13:00420963

Result on the web

<a href="http://dx.doi.org/10.1016/j.cca.2013.05.012" target="_blank" >http://dx.doi.org/10.1016/j.cca.2013.05.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cca.2013.05.012" target="_blank" >10.1016/j.cca.2013.05.012</a>

Result language

angličtina

Original language name

SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses

Original language description

Background: The EC-funded project SPIDIA is aimed to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for DNA molecular testing. To this purpose, a survey and a pan-European External Quality Assessment (EQA) were implemented. Methods: SPIDIA facility sent to all the participants the same blood sample to be processed without time or temperature limitation. DNA quality parameters performed at SPIDIA facility included: UV spectrophotometric analysis of DNA purityand yield, PCR interferences study by Kineret software and DNA integrity analysis by pulsed field gel electrophoresis. Results: 197 applications have been collected from 30 European countries. A high variability of DNA fragmentation was observed whereaspurity, yield and PCR interferences had a narrow distribution within laboratories. A significant difference between the RNase P single copy gene quantity obtained in the DNA samples extracted with the precipitation-based method respect t

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinica Chimica Acta

ISSN

0009-8981

e-ISSN

—

Volume of the periodical

—

Issue of the periodical within the volume

424

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

274-286

UT code for WoS article

000325042600050

EID of the result in the Scopus database

—