Nové mechanismy genové regulace pomocí (super)enhancerů a 3D organizace genomu v krvetvorné diferenciaci

Název projektu anglicky
Novel mechanisms of gene regulation via (super)enhancers and spatial chromatin organization during blood lineage differentiation
Anotace anglicky
Superenhancers are clusters of densely spaced distal gene regulatory elements that were recently discovered to play major role in activation of cell/tissue specifying genes and lineage commitment. They are involved in the interaction of genomic regions necessary for proper gene activation and their disruption can contribute to carcinogenesis. PU.1 is a transcription factor absolutely required for normal hematopoiesis. Precise levels of PU.1 expression stimulate differentiation of hematopoietic cells into various blood lines while its even modest deregulation leads to leukemogenesis. This project will study novel mechanisms of gene regulation mediated by a cooperation of multiple gene regulatory sequences - superenhancers and impact of 3D chromatin interaction during PU.1 dependent hematopoietic differentiation and leukemogenesis. The project is based on deep analysis of regulatory sequences, in vitro modeling, assembly, mutagenesis and functional testing of individual superenhancer constituents, as well as analysis of 3D DNA structure upon differentiation using state of art methods.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10601 - Cell biology
OECD FORD - vedlejší obor
—
OECD FORD - další vedlejší obor
—
CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
EA - Morfologické obory a cytologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP24-GA0-GA-R
Datum dodání záznamu
21. 5. 2024

Podobné projekty(10)