Role guaninových kvadruplexů v architektuře chromatinu zprostředkované interakcí s proteinem CTCF

Název projektu anglicky
Role of guanine quadruplexes in chromatin architecture mediated by CTCF protein
Anotace anglicky
Regulation of gene expression in Eukaryotes, mediated by promoter-enhancer interactions, is partially realized by organization of these regulatory elements into so called topologically-associating domains (TAD). The probability of the interaction and, subsequently, the employment of the enhancer regulatory machinery, is significantly higher within one TAD than within different ones. Key component of the boundaries between individual TADs is the CTCF protein bound to cohesin complex. Recently it was observed using bioinformatics tools that the TAD boundaries well correlate with the position of potential quadruplex-forming sites (G4). G4 are secondary structures of nucleic acids, based on pairing of four guanine residues through Hoogsteen hydrogen bonds. G4 existence in vivo was recently confirmed. In the project, we will study the interaction of different G4 types with CTCF protein and its variants by following direct interaction of purified molecules, using molecular biology and biophysics methods, and by using reporter systems in cell-based models.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10608 - Biochemistry and molecular biology
OECD FORD - vedlejší obor
—
OECD FORD - další vedlejší obor
—
CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
CE - Biochemie<br>EB - Genetika a molekulární biologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP24-GA0-GA-R
Datum dodání záznamu
19. 2. 2024

Podobné projekty(10)