Molekulární podstata biogeneze mikroRNA pomocí komplexu RISC-loading

Název projektu anglicky
Molecular basis of microRNA biogenesis by RISC-loading complex
Anotace anglicky
Silencing of mammalian genes is mediated by microRNAs (miRNA), which are involved in many biological functions ranging from development to cell proliferation and cancer. A key step in the miRNA pathway is the processing and assembly of miRNAs into a large ribonucleoprotein complex called RISC-loading complex. Models for the RISC loading process have emerged, but the structural basis of the process has not been determined. We aim to capture the critical steps of miRNA biogenesis through the RISC loading process. Building on our previous studies in which we captured Dicer in substrate selection and cleavage states, we will now study the post-cleavage states and how these states are modulated by regulatory proteins. We will elucidate the mechanism by which the double-stranded miRNA is passed from Dicer to Argonaut, how one strand is selected to become the mature miRNA, and how a correctly loaded RISC is released from the RISC loading complex. Overall, this study will identify key features of the RLC mechanism that may be exploited for therapeutic purposes.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10614 - Behavioral sciences biology
OECD FORD - vedlejší obor
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OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
EG - Zoologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP25-GA0-GA-R
Datum dodání záznamu
21. 2. 2025

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