Detection of donor DNA after heart transplantation: how far could it be affected by blood transfusion and donor chimerism?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F07%3A00000783" target="_blank" >RIV/00023001:_____/07:00000783 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of donor DNA after heart transplantation: how far could it be affected by blood transfusion and donor chimerism?

Popis výsledku v původním jazyce

The possibility to detect donor DNA in recipient plasma has been discussed as a method to prove organ rejection without a biopsy. Usually, the presence of Y chromosome-specific DNA sequences in female recipients has been used as an example. We have analyzed the presence of part of Y chromosomes in females after heart transplantation. The results suggested that pretransplantation blood transfusion together with cell chimerism of donor organs could be factors that affect detection of donor DNA in recipient plasma. Among females who have undergone transplantation, if the donor organ is chimeric, nested polymerase chain reaction may permit detection of Y chromosome-specific DNA sequences to estimate rejection. In other cases, extremely well-controlled methods using multiple markers need to be developed to avoid the danger of false-positive or false-negative results.

Název v anglickém jazyce

Detection of donor DNA after heart transplantation: how far could it be affected by blood transfusion and donor chimerism?

Popis výsledku anglicky

The possibility to detect donor DNA in recipient plasma has been discussed as a method to prove organ rejection without a biopsy. Usually, the presence of Y chromosome-specific DNA sequences in female recipients has been used as an example. We have analyzed the presence of part of Y chromosomes in females after heart transplantation. The results suggested that pretransplantation blood transfusion together with cell chimerism of donor organs could be factors that affect detection of donor DNA in recipient plasma. Among females who have undergone transplantation, if the donor organ is chimeric, nested polymerase chain reaction may permit detection of Y chromosome-specific DNA sequences to estimate rejection. In other cases, extremely well-controlled methods using multiple markers need to be developed to avoid the danger of false-positive or false-negative results.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FA - Kardiovaskulární nemoci včetně kardiochirurgie

OECD FORD obor

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Projekt

<a href="/cs/project/NR8547" target="_blank" >NR8547: Diagnostika akutní rejekce u pacientů po ortotopické transplantaci srdce pomocí detekce dárcovské DNA v séru příjemce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transplantation Proceedings

ISSN

0041-1345

e-ISSN

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Svazek periodika

39

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

1593-1595

Kód UT WoS článku

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EID výsledku v databázi Scopus

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