Assessment of mitochondrial DNA as an indicator of islet quality: an example in Goto Kakizaki rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F11%3A00002528" target="_blank" >RIV/00023001:_____/11:00002528 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/11:00370179

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0041134511013340" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0041134511013340</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.transproceed.2011.09.055" target="_blank" >10.1016/j.transproceed.2011.09.055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of mitochondrial DNA as an indicator of islet quality: an example in Goto Kakizaki rats

Popis výsledku v původním jazyce

BACKGROUND: Diabetic Goto Kakizaki (GK) rats represent an established model of type 2 diabetes that exhibit an onset of pancreatic islet (PI) pathology characterized by islet hypertrophy with a decreased number of insulin-secreting ?-cells. Among the remaining ?-cells, oxidative phosphorylation (OXPHOS) and consequently glucose-stimulated insulin secretion (GSIS) are impaired, perhaps owing to a deficit in mitochondrial DNA (mtDNA). We sought to identify this abnormality. METHODS: ?-Cells were obtainedfrom Accutase-dissolved PI isolated from GK or Wistar rats and sorted based on the positive Zn(2+) signal of Newport Green. The mtDNA copy number per cell was quantified as the amplicon ratio by polymerase chain reaction using specific primers against the rat ND5 mt gene and UCP2 nuclear gene. RESULTS: The 12-month-old GK rats exhibited drastically reduced copy numbers per remaining ?-cell, from 7,400 ? 600 in 12-month old Wistar rats (100%) to 24 ? 4%; mtDNA content in heart and liver w

Název v anglickém jazyce

Assessment of mitochondrial DNA as an indicator of islet quality: an example in Goto Kakizaki rats

Popis výsledku anglicky

BACKGROUND: Diabetic Goto Kakizaki (GK) rats represent an established model of type 2 diabetes that exhibit an onset of pancreatic islet (PI) pathology characterized by islet hypertrophy with a decreased number of insulin-secreting ?-cells. Among the remaining ?-cells, oxidative phosphorylation (OXPHOS) and consequently glucose-stimulated insulin secretion (GSIS) are impaired, perhaps owing to a deficit in mitochondrial DNA (mtDNA). We sought to identify this abnormality. METHODS: ?-Cells were obtainedfrom Accutase-dissolved PI isolated from GK or Wistar rats and sorted based on the positive Zn(2+) signal of Newport Green. The mtDNA copy number per cell was quantified as the amplicon ratio by polymerase chain reaction using specific primers against the rat ND5 mt gene and UCP2 nuclear gene. RESULTS: The 12-month-old GK rats exhibited drastically reduced copy numbers per remaining ?-cell, from 7,400 ? 600 in 12-month old Wistar rats (100%) to 24 ? 4%; mtDNA content in heart and liver w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

<a href="/cs/project/NS10219" target="_blank" >NS10219: Respirace, oxidační stres a morfologie mitochondrií Langerhansových ostrůvků určených pro transplantace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transplantation Proceedings

ISSN

0041-1345

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

3281-3284

Kód UT WoS článku

000297485500034

EID výsledku v databázi Scopus

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