HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F14%3A00059063" target="_blank" >RIV/00023001:_____/14:00059063 - isvavai.cz</a>

Výsledek na webu

<a href="http://fb.cuni.cz/file/5725/fb2014a0017.pdf" target="_blank" >http://fb.cuni.cz/file/5725/fb2014a0017.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

Popis výsledku v původním jazyce

OTC encodes ornithine carbamoyltransferase, mitochondrial matrix enzyme involved in the synthesis of urea. The tissue-specific expression of OTC in the liver and intestine is dependent on the interaction of OTC promoter with an upstream enhancer. HNF-4 and C/EBP beta are crucial for this interaction in the rat and mouse. In the present study we focused on characterization of elements involved in the regulation of OTC transcription in human. Using a set of 5'-deleted promoter mutants in a reporter assaywe identified two positive cis-acting regulatory elements located at c.-105 and c.-136 within the human OTC promoter. Both are essential for the transcriptional activity of the promoter itself and for the interaction with the enhancer. Protein binding atthe corresponding sites was confirmed by DNase I footprinting. Electromobility shift assay with a specific competitor and anti-HNF-4 alpha antibody identified the DNA-protein binding sites as HNF-4 alpha recognition motifs. A third IINF-

Název v anglickém jazyce

HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

Popis výsledku anglicky

OTC encodes ornithine carbamoyltransferase, mitochondrial matrix enzyme involved in the synthesis of urea. The tissue-specific expression of OTC in the liver and intestine is dependent on the interaction of OTC promoter with an upstream enhancer. HNF-4 and C/EBP beta are crucial for this interaction in the rat and mouse. In the present study we focused on characterization of elements involved in the regulation of OTC transcription in human. Using a set of 5'-deleted promoter mutants in a reporter assaywe identified two positive cis-acting regulatory elements located at c.-105 and c.-136 within the human OTC promoter. Both are essential for the transcriptional activity of the promoter itself and for the interaction with the enhancer. Protein binding atthe corresponding sites was confirmed by DNase I footprinting. Electromobility shift assay with a specific competitor and anti-HNF-4 alpha antibody identified the DNA-protein binding sites as HNF-4 alpha recognition motifs. A third IINF-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FE - Ostatní obory vnitřního lékařství

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia biologica (Praha)

ISSN

0015-5500

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

133-143

Kód UT WoS článku

000338479400005

EID výsledku v databázi Scopus

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