In vivo monitoring of rat macrophages labeled with poly(L-lysine)-iron oxide nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F15%3A00059491" target="_blank" >RIV/00023001:_____/15:00059491 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/15:00446014 RIV/61389013:_____/15:00446014

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33292/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33292/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbm.b.33292" target="_blank" >10.1002/jbm.b.33292</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo monitoring of rat macrophages labeled with poly(L-lysine)-iron oxide nanoparticles

Popis výsledku v původním jazyce

Coprecipitation of FeCl2 and FeCl3 with aqueous ammonia was used to prepare iron oxide nanoparticles dispersible in aqueous medium. Oxidation of the particles with sodium hypochlorite then yielded maghemite (g-Fe2O3) nanoparticles which were coated with two types of coating -D-mannose or poly(L-lysine) (PLL) as confirmed by FTIR analysis. The particles were <10 nm according to transmission electron microscopy. Their hydrodynamic particle size was 180 nm (by dynamic light scattering). The D-mannose-, PLL-coated, and neat g-Fe2O3 particles as well as commercial ResovistVR were used to label rat macrophages. The viability and contrast properties of labeled macrophages were compared. PLL-coated g-Fe2O3 nanoparticles were found optimal. The labeled macrophages were injected to rats monitored in vivo by magnetic resonance imaging up to 48 h. Transport of macrophages labeled with PLL-g-Fe2O3 nanoparticles in rats was confirmed. Tracking of macrophages using the developed particles can be used formonitoring of inflammations and cell migration in cell therapy.

Název v anglickém jazyce

In vivo monitoring of rat macrophages labeled with poly(L-lysine)-iron oxide nanoparticles

Popis výsledku anglicky

Coprecipitation of FeCl2 and FeCl3 with aqueous ammonia was used to prepare iron oxide nanoparticles dispersible in aqueous medium. Oxidation of the particles with sodium hypochlorite then yielded maghemite (g-Fe2O3) nanoparticles which were coated with two types of coating -D-mannose or poly(L-lysine) (PLL) as confirmed by FTIR analysis. The particles were <10 nm according to transmission electron microscopy. Their hydrodynamic particle size was 180 nm (by dynamic light scattering). The D-mannose-, PLL-coated, and neat g-Fe2O3 particles as well as commercial ResovistVR were used to label rat macrophages. The viability and contrast properties of labeled macrophages were compared. PLL-coated g-Fe2O3 nanoparticles were found optimal. The labeled macrophages were injected to rats monitored in vivo by magnetic resonance imaging up to 48 h. Transport of macrophages labeled with PLL-g-Fe2O3 nanoparticles in rats was confirmed. Tracking of macrophages using the developed particles can be used formonitoring of inflammations and cell migration in cell therapy.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of biomedical materials research. Part B, Applied biomaterials

ISSN

1552-4973

e-ISSN

—

Svazek periodika

103

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1141-1148

Kód UT WoS článku

000358484700001

EID výsledku v databázi Scopus

—