D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00507478" target="_blank" >RIV/61389013:_____/19:00507478 - isvavai.cz</a>
Výsledek na webu
<a href="https://journals.sagepub.com/doi/pdf/10.1177/0963689719834304" target="_blank" >https://journals.sagepub.com/doi/pdf/10.1177/0963689719834304</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/0963689719834304" target="_blank" >10.1177/0963689719834304</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain
Popis výsledku v původním jazyce
Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe2O3)) in neural stem cells (NSCs) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSCs transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D-mannose(γ-Fe2O3) labeled the NSCs better than the uncoated nanoparticles. The labeled cells were visualized by ex vivo MRI and their localization subsequently confirmed on histological sections. Although the progenitor properties and differentiation of the NSCs were not affected by labeling, subtle effects on stem cells could be detected depending on dose increase, including changes in cell proliferation, viability, and neurosphere diameter. D-mannose coating of maghemite nanoparticles improved NSC labeling and allowed for NSC tracking by ex vivo MRI in the mouse brain, but further analysis of the eventual side effects might be necessary before translation to the clinic.
Název v anglickém jazyce
D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain
Popis výsledku anglicky
Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe2O3)) in neural stem cells (NSCs) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSCs transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D-mannose(γ-Fe2O3) labeled the NSCs better than the uncoated nanoparticles. The labeled cells were visualized by ex vivo MRI and their localization subsequently confirmed on histological sections. Although the progenitor properties and differentiation of the NSCs were not affected by labeling, subtle effects on stem cells could be detected depending on dose increase, including changes in cell proliferation, viability, and neurosphere diameter. D-mannose coating of maghemite nanoparticles improved NSC labeling and allowed for NSC tracking by ex vivo MRI in the mouse brain, but further analysis of the eventual side effects might be necessary before translation to the clinic.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
<a href="/cs/project/LQ1604" target="_blank" >LQ1604: BIOCEV - od základního k aplikovanému výzkumu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cell Transplantation
ISSN
0963-6897
e-ISSN
—
Svazek periodika
28
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
15
Strana od-do
553-567
Kód UT WoS článku
000477514700001
EID výsledku v databázi Scopus
2-s2.0-85070113811