Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7T

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F16%3A00060053" target="_blank" >RIV/00023001:_____/16:00060053 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.omicsonline.org/open-access/magnetic-resonance-visualization-of-pancreatic-islets-labeled-by-paracest-contrast-agents-at-47-t-2155-9937-1000121.php?aid=68387" target="_blank" >http://www.omicsonline.org/open-access/magnetic-resonance-visualization-of-pancreatic-islets-labeled-by-paracest-contrast-agents-at-47-t-2155-9937-1000121.php?aid=68387</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4172/2155-9937.1000121" target="_blank" >10.4172/2155-9937.1000121</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7T

Popis výsledku v původním jazyce

Introduction: Monitoring of Pancreatic Islets (PIs) after their transplantation could provide important data related to diabetes treatment; however their visualization is conditioned by the contrast agent use. The aim of this study was to label and visualize PIs labeled by the contrast agents based on Chemical Exchange Saturation Transfer (CEST) owing an advantage of contrast switching on/off and simultaneous visualization of differently treated cell populations in one MR experiment. Material and methods: Two paramagnetic chelates with europium Eu-DO3A-ae and ytterbium Yb-DO3A-ae were tested for labeling of PIs by pinocytosis and microporation. The toxicity, labeling efficacy and detection threshold were assessed for each agent. In vitro MR imaging of the labeled islets was performed on a 4.7 T scanner using a modified turbo spin-echo (RARE) sequence. Results: The sufficient labeling efficacy was observed only by using high agent concentration compromising islet viability. The microporation procedure was not effective for islet labeling because of its invasive nature. The islets labeled by pinocytosis were visualized in a phantom by CEST imaging, however, after a long acquisition time at 4.7 T. Conclusion: The low sensitivity of detection and impaired cellular viability of the pancreatic islets labeled by the novel CEST contrast agents represent a challenge for the further implementation of these probes as exogenous cellular labels.

Název v anglickém jazyce

Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7T

Popis výsledku anglicky

Introduction: Monitoring of Pancreatic Islets (PIs) after their transplantation could provide important data related to diabetes treatment; however their visualization is conditioned by the contrast agent use. The aim of this study was to label and visualize PIs labeled by the contrast agents based on Chemical Exchange Saturation Transfer (CEST) owing an advantage of contrast switching on/off and simultaneous visualization of differently treated cell populations in one MR experiment. Material and methods: Two paramagnetic chelates with europium Eu-DO3A-ae and ytterbium Yb-DO3A-ae were tested for labeling of PIs by pinocytosis and microporation. The toxicity, labeling efficacy and detection threshold were assessed for each agent. In vitro MR imaging of the labeled islets was performed on a 4.7 T scanner using a modified turbo spin-echo (RARE) sequence. Results: The sufficient labeling efficacy was observed only by using high agent concentration compromising islet viability. The microporation procedure was not effective for islet labeling because of its invasive nature. The islets labeled by pinocytosis were visualized in a phantom by CEST imaging, however, after a long acquisition time at 4.7 T. Conclusion: The low sensitivity of detection and impaired cellular viability of the pancreatic islets labeled by the novel CEST contrast agents represent a challenge for the further implementation of these probes as exogenous cellular labels.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular Imaging and Dynamics

ISSN

2155-9937

e-ISSN

—

Svazek periodika

2016

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—