Gadolinium- and Manganite-Based Contrast Agents with Fluorescent Probes for Both Magnetic Resonance and Fluorescence Imaging of Pancreatic Islets: A Comparative Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10139596" target="_blank" >RIV/00216208:11110/13:10139596 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/13:00425115 RIV/00216208:11310/13:10139596 RIV/00023001:_____/13:00058541

Výsledek na webu

<a href="http://dx.doi.org/10.1002/cmdc.201200439" target="_blank" >http://dx.doi.org/10.1002/cmdc.201200439</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cmdc.201200439" target="_blank" >10.1002/cmdc.201200439</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gadolinium- and Manganite-Based Contrast Agents with Fluorescent Probes for Both Magnetic Resonance and Fluorescence Imaging of Pancreatic Islets: A Comparative Study

Popis výsledku v původním jazyce

Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets invitro and following transplantation. As T1 contrast agents (CAs), gadolinium(III) complexes linkedto -cyclodextrin (Gd-F-CD) or bound to titanium dioxide (TiO2@RhdGd) were tested. As a T2 CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeledwith gadolinium(III) CAs were visible as hyperintense spots on MR invitro, but detection invivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans invitro as well as invivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling, as it provid

Název v anglickém jazyce

Gadolinium- and Manganite-Based Contrast Agents with Fluorescent Probes for Both Magnetic Resonance and Fluorescence Imaging of Pancreatic Islets: A Comparative Study

Popis výsledku anglicky

Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets invitro and following transplantation. As T1 contrast agents (CAs), gadolinium(III) complexes linkedto -cyclodextrin (Gd-F-CD) or bound to titanium dioxide (TiO2@RhdGd) were tested. As a T2 CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeledwith gadolinium(III) CAs were visible as hyperintense spots on MR invitro, but detection invivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans invitro as well as invivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling, as it provid

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CA - Anorganická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP207%2F11%2F1437" target="_blank" >GAP207/11/1437: Nové třídy kontrastních látek pro tomografii magnetické rezonance</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemMedChem

ISSN

1860-7179

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

614-621

Kód UT WoS článku

000316811100010

EID výsledku v databázi Scopus

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