Steroid free immunosuppression is associated with enhanced Th1 transcripts in kidney transplantation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F17%3A00075891" target="_blank" >RIV/00023001:_____/17:00075891 - isvavai.cz</a>

Výsledek na webu

<a href="http://ac.els-cdn.com/S0966327416301435/1-s2.0-S0966327416301435-main.pdf?_tid=ce946338-8727-11e7-af5c-00000aab0f26&acdnat=1503399249_1ce5de1ebda8228de9285e20615158e6" target="_blank" >http://ac.els-cdn.com/S0966327416301435/1-s2.0-S0966327416301435-main.pdf?_tid=ce946338-8727-11e7-af5c-00000aab0f26&acdnat=1503399249_1ce5de1ebda8228de9285e20615158e6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.trim.2017.03.001" target="_blank" >10.1016/j.trim.2017.03.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Steroid free immunosuppression is associated with enhanced Th1 transcripts in kidney transplantation

Popis výsledku v původním jazyce

Background: Steroid avoidance in immunosuppression in kidney transplantation offers several metabolic advantages, however it is associated with higher early acute rejection rate. Cellular and molecular mechanisms of this phenomenon remain poorly understood. Methods: In this single center observational study, low-risk kidney transplant recipients randomized into large multicenter prospective ADVANCE trial with steroid avoidance/early withdrawal and center standard of care treated patients were monitored for 12 months. The expressions of 28 transcripts, associated with alloimmune response and operational tolerance, were evaluated in the peripheral blood using RT-qPCR at 0, 7, 14, 90 and 365 postoperative days (POD) and in the protocol graft biopsy at 3 months while lymphocyte subpopulations were analyzed by flow-cytometry within the follow-up. Results: Both steroid avoidance and withdrawal regimens were associated with significantly higher granzyme B (GZMB) transcript at POD 14 and perforin 1 (PRF1) transcript at POD 7. The higher interleukin 2 (IL-2) expression at POD 7 was detected only in the steroid avoidance group. Initial steroids decreased the expression SH2D1B transcript at POD14 and there were no further differences in other operational tolerance transcripts among groups. The statistically significant decrease in absolute numbers of peripheral NK cells in the first 14 days was observed in the standard of care group only. There were no differences in analyzed intrarenal transcripts in 3-month biopsies among groups. Conclusions: The enhanced expression of some of Th1 associated transcripts and limited effects on NK cells of steroid avoidance immunosuppression suggest higher susceptibility for early acute rejection.

Název v anglickém jazyce

Steroid free immunosuppression is associated with enhanced Th1 transcripts in kidney transplantation

Popis výsledku anglicky

Background: Steroid avoidance in immunosuppression in kidney transplantation offers several metabolic advantages, however it is associated with higher early acute rejection rate. Cellular and molecular mechanisms of this phenomenon remain poorly understood. Methods: In this single center observational study, low-risk kidney transplant recipients randomized into large multicenter prospective ADVANCE trial with steroid avoidance/early withdrawal and center standard of care treated patients were monitored for 12 months. The expressions of 28 transcripts, associated with alloimmune response and operational tolerance, were evaluated in the peripheral blood using RT-qPCR at 0, 7, 14, 90 and 365 postoperative days (POD) and in the protocol graft biopsy at 3 months while lymphocyte subpopulations were analyzed by flow-cytometry within the follow-up. Results: Both steroid avoidance and withdrawal regimens were associated with significantly higher granzyme B (GZMB) transcript at POD 14 and perforin 1 (PRF1) transcript at POD 7. The higher interleukin 2 (IL-2) expression at POD 7 was detected only in the steroid avoidance group. Initial steroids decreased the expression SH2D1B transcript at POD14 and there were no further differences in other operational tolerance transcripts among groups. The statistically significant decrease in absolute numbers of peripheral NK cells in the first 14 days was observed in the standard of care group only. There were no differences in analyzed intrarenal transcripts in 3-month biopsies among groups. Conclusions: The enhanced expression of some of Th1 associated transcripts and limited effects on NK cells of steroid avoidance immunosuppression suggest higher susceptibility for early acute rejection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30101 - Human genetics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transplant immunology

ISSN

0966-3274

e-ISSN

—

Svazek periodika

42

Číslo periodika v rámci svazku

June 2017

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

18-23

Kód UT WoS článku

000403626200004

EID výsledku v databázi Scopus

—