Low-molecular-weight paramagnetic F-19 contrast agents for fluorine magnetic resonance imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00077807" target="_blank" >RIV/00023001:_____/19:00077807 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10393766 RIV/00216208:11110/19:10393766 RIV/00216208:11320/19:10393766

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007%2Fs10334-018-0721-9.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs10334-018-0721-9.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10334-018-0721-9" target="_blank" >10.1007/s10334-018-0721-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low-molecular-weight paramagnetic F-19 contrast agents for fluorine magnetic resonance imaging

Popis výsledku v původním jazyce

Objective(19)F MRI requires biocompatible and non-toxic soluble contrast agents with high fluorine content and with suitable F-19 relaxation times. Probes based on a DOTP chelate with 12 magnetically equivalent fluorine atoms (DOTP-tfe) and a lanthanide(III) ion shortening the relaxation times were prepared and tested.MethodsComplexes of DOTP-tfe with trivalent paramagnetic Ce, Dy, Ho, Tm, and Yb ions were synthetized and characterized. F-19 relaxation times were determined and compared to those of the La complex and of the empty ligand. In vitro and in vivo F-19 MRI was performed at 4.7T.Results(19)F relaxation times strongly depended on the chelated lanthanide(III) ion. T-1 ranged from 6.5 to 287ms, T-2 from 3.9 to 124.4ms, and T-2* from 1.1 to 3.1ms. All complexes in combination with optimized sequences provided sufficient signal in vitro under conditions mimicking experiments in vivo (concentrations 1.25mM, 15-min scanning time). As a proof of concept, two contrast agents were injected into the rat muscle; F-19 MRI in vivo confirmed the in vivo applicability of the probe.ConclusionDOTP-based F-19 probes showed suitable properties for in vitro and in vivo visualization and biological applications. The lanthanide(III) ions enabled us to shorten the relaxation times and to trim the probes according to the actual needs. Similar to the clinically approved Gd3+ chelates, this customized probe design ensures consistent biochemical properties and similar safety profiles.

Název v anglickém jazyce

Low-molecular-weight paramagnetic F-19 contrast agents for fluorine magnetic resonance imaging

Popis výsledku anglicky

Objective(19)F MRI requires biocompatible and non-toxic soluble contrast agents with high fluorine content and with suitable F-19 relaxation times. Probes based on a DOTP chelate with 12 magnetically equivalent fluorine atoms (DOTP-tfe) and a lanthanide(III) ion shortening the relaxation times were prepared and tested.MethodsComplexes of DOTP-tfe with trivalent paramagnetic Ce, Dy, Ho, Tm, and Yb ions were synthetized and characterized. F-19 relaxation times were determined and compared to those of the La complex and of the empty ligand. In vitro and in vivo F-19 MRI was performed at 4.7T.Results(19)F relaxation times strongly depended on the chelated lanthanide(III) ion. T-1 ranged from 6.5 to 287ms, T-2 from 3.9 to 124.4ms, and T-2* from 1.1 to 3.1ms. All complexes in combination with optimized sequences provided sufficient signal in vitro under conditions mimicking experiments in vivo (concentrations 1.25mM, 15-min scanning time). As a proof of concept, two contrast agents were injected into the rat muscle; F-19 MRI in vivo confirmed the in vivo applicability of the probe.ConclusionDOTP-based F-19 probes showed suitable properties for in vitro and in vivo visualization and biological applications. The lanthanide(III) ions enabled us to shorten the relaxation times and to trim the probes according to the actual needs. Similar to the clinically approved Gd3+ chelates, this customized probe design ensures consistent biochemical properties and similar safety profiles.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Magnetic resonance materials in physics, biology and medicine

ISSN

0968-5243

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

115-122

Kód UT WoS článku

000460767000012

EID výsledku v databázi Scopus

2-s2.0-85057583208