Dendritic cells in subcutaneous and epicardial adipose tissue of subjects with type 2 diabetes, obesity, and coronary artery disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078037" target="_blank" >RIV/00023001:_____/19:00078037 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10394874 RIV/00064165:_____/19:10394874

Výsledek na webu

<a href="https://www.hindawi.com/journals/mi/2019/5481725/" target="_blank" >https://www.hindawi.com/journals/mi/2019/5481725/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2019/5481725" target="_blank" >10.1155/2019/5481725</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dendritic cells in subcutaneous and epicardial adipose tissue of subjects with type 2 diabetes, obesity, and coronary artery disease

Popis výsledku v původním jazyce

Dendritic cells (DCs) are professional antigen-presenting cells contributing to regulation of lymphocyte immune response. DCs are divided into two subtypes: CD11c-positive conventional or myeloid (cDCs) and CD123-positive plasmacytoid (pDCs) DCs. The aim of the study was to assess DCs (HLA-DR+ lineage-) and their subtypes by flow cytometry in peripheral blood and subcutaneous (SAT) and epicardial (EAT) adipose tissue in subjects with (T2DM, n=12) and without (non-T2DM, n=17) type 2 diabetes mellitus undergoing elective cardiac surgery. Subjects with T2DM had higher fasting glycemia (8.6 +/- 0.7 vs. 5.8 +/- 0.2mmol/l, p&lt;0.001) and glycated hemoglobin (52.0 +/- 3.4 vs. 36.9 +/- 1.0mmol/mol, p&lt;0.001) and tended to have more pronounced inflammation (hsCRP: 9.8 +/- 3.1 vs. 5.1 +/- 1.9mg/ml, p=0.177) compared with subjects without T2DM. T2DM was associated with reduced total DCs in SAT (1.57 +/- 0.65 vs. 4.45 +/- 1.56% for T2DM vs. non-T2DM, p=0.041) with a similar, albeit insignificant, trend in EAT (0.996 +/- 0.33 vs. 2.46 +/- 0.78% for T2DM vs. non-T2DM, p=0.171). When analyzing DC subsets, no difference in cDCs was seen between any of the studied groups or adipose tissue pools. In contrast, pDCs were increased in both SAT (13.5 +/- 2.0 vs. 4.6 +/- 1.9% of DC cells, p=0.005) and EAT (29.1 +/- 8.7 vs. 8.4 +/- 2.4% of DC, p=0.045) of T2DM relative to non-T2DM subjects as well as in EAT of the T2DM group compared with corresponding SAT (29.1 +/- 8.7 vs. 13.5 +/- 2.0% of DC, p=0.020). Neither obesity nor coronary artery disease (CAD) significantly influenced the number of total, cDC, or pDC in SAT or EAT according to multiple regression analysis. In summary, T2DM decreased the amount of total dendritic cells in subcutaneous adipose tissue and increased plasmacytoid dendritic cells in subcutaneous and even more in epicardial adipose tissue. These findings suggest a potential role of pDCs in the development of T2DM-associated adipose tissue low-grade inflammation.

Název v anglickém jazyce

Dendritic cells in subcutaneous and epicardial adipose tissue of subjects with type 2 diabetes, obesity, and coronary artery disease

Popis výsledku anglicky

Dendritic cells (DCs) are professional antigen-presenting cells contributing to regulation of lymphocyte immune response. DCs are divided into two subtypes: CD11c-positive conventional or myeloid (cDCs) and CD123-positive plasmacytoid (pDCs) DCs. The aim of the study was to assess DCs (HLA-DR+ lineage-) and their subtypes by flow cytometry in peripheral blood and subcutaneous (SAT) and epicardial (EAT) adipose tissue in subjects with (T2DM, n=12) and without (non-T2DM, n=17) type 2 diabetes mellitus undergoing elective cardiac surgery. Subjects with T2DM had higher fasting glycemia (8.6 +/- 0.7 vs. 5.8 +/- 0.2mmol/l, p&lt;0.001) and glycated hemoglobin (52.0 +/- 3.4 vs. 36.9 +/- 1.0mmol/mol, p&lt;0.001) and tended to have more pronounced inflammation (hsCRP: 9.8 +/- 3.1 vs. 5.1 +/- 1.9mg/ml, p=0.177) compared with subjects without T2DM. T2DM was associated with reduced total DCs in SAT (1.57 +/- 0.65 vs. 4.45 +/- 1.56% for T2DM vs. non-T2DM, p=0.041) with a similar, albeit insignificant, trend in EAT (0.996 +/- 0.33 vs. 2.46 +/- 0.78% for T2DM vs. non-T2DM, p=0.171). When analyzing DC subsets, no difference in cDCs was seen between any of the studied groups or adipose tissue pools. In contrast, pDCs were increased in both SAT (13.5 +/- 2.0 vs. 4.6 +/- 1.9% of DC cells, p=0.005) and EAT (29.1 +/- 8.7 vs. 8.4 +/- 2.4% of DC, p=0.045) of T2DM relative to non-T2DM subjects as well as in EAT of the T2DM group compared with corresponding SAT (29.1 +/- 8.7 vs. 13.5 +/- 2.0% of DC, p=0.020). Neither obesity nor coronary artery disease (CAD) significantly influenced the number of total, cDC, or pDC in SAT or EAT according to multiple regression analysis. In summary, T2DM decreased the amount of total dendritic cells in subcutaneous adipose tissue and increased plasmacytoid dendritic cells in subcutaneous and even more in epicardial adipose tissue. These findings suggest a potential role of pDCs in the development of T2DM-associated adipose tissue low-grade inflammation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/NV15-26854A" target="_blank" >NV15-26854A: Význam epikardiální tukové tkáně u onemocnění srdce: nové mechanizmy působení a jejich terapeutické ovlivnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mediators of inflammation [online]

ISSN

0962-9351

e-ISSN

—

Svazek periodika

2019

Číslo periodika v rámci svazku

May 9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

"art. no. 5481725"

Kód UT WoS článku

000468514100001

EID výsledku v databázi Scopus

2-s2.0-85068368035