Origin of the (31) P MR signal at 5.3 ppm in patients with critical limb ischemia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F20%3A00079645" target="_blank" >RIV/00023001:_____/20:00079645 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.4295" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.4295</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/nbm.4295" target="_blank" >10.1002/nbm.4295</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Origin of the (31) P MR signal at 5.3 ppm in patients with critical limb ischemia

Popis výsledku v původním jazyce

An unknown intense signal (P(un) ) with a mean chemical shift of 5.3 ppm was observed in (31) P MR spectra from the calf muscles of patients with the diabetic foot syndrome. The aim of the study was to identify the origin of this signal and its potential as a biomarker of muscle injury. Calf muscles of 68 diabetic patients (66.3 ± 8.6 years; body mass index = 28.2 ± 4.3 kg/m(2) ) and 12 age-matched healthy controls were examined by (dynamic) (31) P MRS (3 T system, (31) P/(1) H coil). Phantoms (glucose-1-phosphate, P(i) and PCr) were measured at pH values of 7.05 and 7.51. At rest, P(un) signals with intensities higher than 50% of the P(i) intensity were observed in 10 of the 68 examined diabetic subjects. We tested two hypothetical origins of the P(un) signal: (1) phosphorus from phosphoesters and (2) phosphorus from extra- and intracellular alkaline phosphate pools. 2,3-diphosphoglycerate and glucose-1-phosphate are the only phosphoesters with signals in the chemical shift region close to 5.3 ppm. Both compounds can be excluded: 2,3-diphosphoglycerate due to the missing second signal component at 6.31 ppm; glucose-1-phosphate because its chemical shifts are about 0.2 ppm downfield from the P(i) signal (4.9 ppm). If the P(un) signal is from phosphate, it represents a pH value of 7.54 ± 0.05. Therefore, it could correspond to signals of P(i) in mitochondria. However, patients with critical limb ischemia have rather few mitochondria and so the P(un) signal probably originates from interstitia. Our data suggest that the increased P(un) signal observed in patients with the diabetic foot syndrome is a biomarker of severe muscular damage.

Název v anglickém jazyce

Origin of the (31) P MR signal at 5.3 ppm in patients with critical limb ischemia

Popis výsledku anglicky

An unknown intense signal (P(un) ) with a mean chemical shift of 5.3 ppm was observed in (31) P MR spectra from the calf muscles of patients with the diabetic foot syndrome. The aim of the study was to identify the origin of this signal and its potential as a biomarker of muscle injury. Calf muscles of 68 diabetic patients (66.3 ± 8.6 years; body mass index = 28.2 ± 4.3 kg/m(2) ) and 12 age-matched healthy controls were examined by (dynamic) (31) P MRS (3 T system, (31) P/(1) H coil). Phantoms (glucose-1-phosphate, P(i) and PCr) were measured at pH values of 7.05 and 7.51. At rest, P(un) signals with intensities higher than 50% of the P(i) intensity were observed in 10 of the 68 examined diabetic subjects. We tested two hypothetical origins of the P(un) signal: (1) phosphorus from phosphoesters and (2) phosphorus from extra- and intracellular alkaline phosphate pools. 2,3-diphosphoglycerate and glucose-1-phosphate are the only phosphoesters with signals in the chemical shift region close to 5.3 ppm. Both compounds can be excluded: 2,3-diphosphoglycerate due to the missing second signal component at 6.31 ppm; glucose-1-phosphate because its chemical shifts are about 0.2 ppm downfield from the P(i) signal (4.9 ppm). If the P(un) signal is from phosphate, it represents a pH value of 7.54 ± 0.05. Therefore, it could correspond to signals of P(i) in mitochondria. However, patients with critical limb ischemia have rather few mitochondria and so the P(un) signal probably originates from interstitia. Our data suggest that the increased P(un) signal observed in patients with the diabetic foot syndrome is a biomarker of severe muscular damage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

<a href="/cs/project/NV16-27262A" target="_blank" >NV16-27262A: Srovnání vlivu aplikace autologních kmenových buněk a standardní revaskularizace na oxygenaci tkání a průběh syndromu diabetické nohy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NMR in biomedicine

ISSN

0952-3480

e-ISSN

—

Svazek periodika

33

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

"e4295"

Kód UT WoS článku

000534219200003

EID výsledku v databázi Scopus

2-s2.0-85081722389