The effect of long-term left ventricular assist device support on flow-sensitive plasma microRNA levels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F21%3A00081667" target="_blank" >RIV/00023001:_____/21:00081667 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/21:43921773 RIV/00216208:11110/21:10429102

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0167527321010883?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0167527321010883?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijcard.2021.06.050" target="_blank" >10.1016/j.ijcard.2021.06.050</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of long-term left ventricular assist device support on flow-sensitive plasma microRNA levels

Popis výsledku v původním jazyce

Background: Implantation of current generation left ventricular assist devices (LVADs) in the treatment of end-stage heart failure (HF), not only improves HF symptoms and end-organ perfusion, but also leads to cellular and molecular responses, presumably in response to the continuous flow generated by these devices. MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression in multiple biological processes, including the pathogenesis of HF. In our study, we examined the influence of long-term LVAD support on changes in flow-sensitive miRNAs in plasma. Materials and methods: Blood samples from patients with end-stage heart failure (N = 33; age = 55.7 ± 11.6 years) were collected before LVAD implantation and 3, 6, 9, and 12 months after implantation. Plasma levels of the flow-sensitive miRNAs; miR-10a, miR-10b, miR-146a, miR-146b, miR-663a, miR-663b, miR-21, miR-155, and miR-126 were measured using quantitative PCR. Results: Increasing quantities of miR-126 (P &lt; 0.03) and miR-146a (P &lt; 0.02) was observed at each follow-up visit after LVAD implantation. A positive association between miR-155 and Belcaro score (P &lt; 0.04) and an inverse correlation between miR-126 and endothelial function, measured as the reactive hyperemia index (P &lt; 0.05), was observed. Conclusions: Our observations suggest that after LVAD implantation, low pulsatile flow up-regulates plasma levels of circulating flow-sensitive miRNAs, contributing to endothelial dysfunction and vascular remodeling. © 2021 Elsevier B.V.

Název v anglickém jazyce

The effect of long-term left ventricular assist device support on flow-sensitive plasma microRNA levels

Popis výsledku anglicky

Background: Implantation of current generation left ventricular assist devices (LVADs) in the treatment of end-stage heart failure (HF), not only improves HF symptoms and end-organ perfusion, but also leads to cellular and molecular responses, presumably in response to the continuous flow generated by these devices. MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression in multiple biological processes, including the pathogenesis of HF. In our study, we examined the influence of long-term LVAD support on changes in flow-sensitive miRNAs in plasma. Materials and methods: Blood samples from patients with end-stage heart failure (N = 33; age = 55.7 ± 11.6 years) were collected before LVAD implantation and 3, 6, 9, and 12 months after implantation. Plasma levels of the flow-sensitive miRNAs; miR-10a, miR-10b, miR-146a, miR-146b, miR-663a, miR-663b, miR-21, miR-155, and miR-126 were measured using quantitative PCR. Results: Increasing quantities of miR-126 (P &lt; 0.03) and miR-146a (P &lt; 0.02) was observed at each follow-up visit after LVAD implantation. A positive association between miR-155 and Belcaro score (P &lt; 0.04) and an inverse correlation between miR-126 and endothelial function, measured as the reactive hyperemia index (P &lt; 0.05), was observed. Conclusions: Our observations suggest that after LVAD implantation, low pulsatile flow up-regulates plasma levels of circulating flow-sensitive miRNAs, contributing to endothelial dysfunction and vascular remodeling. © 2021 Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

<a href="/cs/project/NV16-27630A" target="_blank" >NV16-27630A: Markery vaskulárního zdraví u pacientů s implantovanou mechanickou srdeční podporou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International journal of cardiology

ISSN

0167-5273

e-ISSN

—

Svazek periodika

339

Číslo periodika v rámci svazku

15 September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

138-143

Kód UT WoS článku

000722892200037

EID výsledku v databázi Scopus

2-s2.0-85114033245