Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F22%3A00082636" target="_blank" >RIV/00023001:_____/22:00082636 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202100523" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202100523</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mabi.202100523" target="_blank" >10.1002/mabi.202100523</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging

Popis výsledku v původním jazyce

P-31-magnetic resonance (MR) is an important diagnostic technique currently used for tissue metabolites assessing, but it also has great potential for visualizing the internal body structures. However, due to the low physiological level of phosphorus-containing biomolecules, precise imaging requires the administration of an exogenous probe. Herein, this work describes the synthesis and MR characterization of a pioneering metal-free P-31-MR probe based on phosphorus-containing polymeric zwitterion. The developed probe (pTMPC) is a well-defined water-soluble macromolecule characterized by a high content of naturally rare phosphorothioate groups providing a high-intensity P-31-MR signal clearly distinguishable from biological background both in vitro and in vitro. In addition, pTMPC can serve as a sensitive P-31-MR sensor of pathological conditions in vivo because it undergoes oxidation-induced structural changes in the presence of reactive oxygen species (ROS). Add to this the favorable H-1 and P-31 T-1/T-2 relaxation times and biocompatibility, pTMPC represents a conceptually new diagnostic, whose discovery opens up new possibilities in the field of P-31-MR spectroscopy and imaging.

Název v anglickém jazyce

Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging

Popis výsledku anglicky

P-31-magnetic resonance (MR) is an important diagnostic technique currently used for tissue metabolites assessing, but it also has great potential for visualizing the internal body structures. However, due to the low physiological level of phosphorus-containing biomolecules, precise imaging requires the administration of an exogenous probe. Herein, this work describes the synthesis and MR characterization of a pioneering metal-free P-31-MR probe based on phosphorus-containing polymeric zwitterion. The developed probe (pTMPC) is a well-defined water-soluble macromolecule characterized by a high content of naturally rare phosphorothioate groups providing a high-intensity P-31-MR signal clearly distinguishable from biological background both in vitro and in vitro. In addition, pTMPC can serve as a sensitive P-31-MR sensor of pathological conditions in vivo because it undergoes oxidation-induced structural changes in the presence of reactive oxygen species (ROS). Add to this the favorable H-1 and P-31 T-1/T-2 relaxation times and biocompatibility, pTMPC represents a conceptually new diagnostic, whose discovery opens up new possibilities in the field of P-31-MR spectroscopy and imaging.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU20-08-00095" target="_blank" >NU20-08-00095: Nové paradigma pro funkční kontrastní látky pro 31P zobrazování magnetickou rezonancí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Macromolecular bioscience

ISSN

1616-5187

e-ISSN

1616-5195

Svazek periodika

22

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

"Art. no. 2100523"

Kód UT WoS článku

000766947400001

EID výsledku v databázi Scopus

2-s2.0-85126066378