Significant elevations of serum amylase caused by macroamylase : case reports and detection possibilities

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F23%3A00083844" target="_blank" >RIV/00023001:_____/23:00083844 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/23:43925256

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.24859" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.24859</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcla.24859" target="_blank" >10.1002/jcla.24859</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Significant elevations of serum amylase caused by macroamylase : case reports and detection possibilities

Popis výsledku v původním jazyce

BackgroundThe presence of isolated elevated serum amylase levels can be caused by high molecular mass complexes. We describe 13 cases of hyperamylasemia detected in adult patients without clinical symptoms of a pancreatic disorder. Five of them were thoroughly examined using different tools for the detection of macrocomplexes. MethodsWe performed both screening and more advanced methods of macroamylase detection, including polyethylene glycol precipitation, sample storage at 4 degrees C and separation by gel filtration. ResultsThe presence of macroamylase in the suspected samples was confirmed by the methods described, except for the sample storage at 4 degrees C. In this method, the enzyme activity did not decrease. The polyethylene glycol precipitation activity (% PPA) averaged 89.1% for amylase, whereas the control samples averaged 30.7%. Gel filtration chromatography confirmed an IgA macroamylase peak in three samples and an IgG macroamylase peak in two samples. ConclusionThe presence of macroamylase should be suspected whenever the clinical history and condition of the patient do not match the measured enzyme value to avoid diagnostic errors and unnecessary invasive examinations. The presence of macrocomplexes is considered a benign process that may occur in apparently healthy individuals. Cooperation between clinicians and laboratory staff is necessary.

Název v anglickém jazyce

Significant elevations of serum amylase caused by macroamylase : case reports and detection possibilities

Popis výsledku anglicky

BackgroundThe presence of isolated elevated serum amylase levels can be caused by high molecular mass complexes. We describe 13 cases of hyperamylasemia detected in adult patients without clinical symptoms of a pancreatic disorder. Five of them were thoroughly examined using different tools for the detection of macrocomplexes. MethodsWe performed both screening and more advanced methods of macroamylase detection, including polyethylene glycol precipitation, sample storage at 4 degrees C and separation by gel filtration. ResultsThe presence of macroamylase in the suspected samples was confirmed by the methods described, except for the sample storage at 4 degrees C. In this method, the enzyme activity did not decrease. The polyethylene glycol precipitation activity (% PPA) averaged 89.1% for amylase, whereas the control samples averaged 30.7%. Gel filtration chromatography confirmed an IgA macroamylase peak in three samples and an IgG macroamylase peak in two samples. ConclusionThe presence of macroamylase should be suspected whenever the clinical history and condition of the patient do not match the measured enzyme value to avoid diagnostic errors and unnecessary invasive examinations. The presence of macrocomplexes is considered a benign process that may occur in apparently healthy individuals. Cooperation between clinicians and laboratory staff is necessary.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of clinical laboratory analysis

ISSN

0887-8013

e-ISSN

1098-2825

Svazek periodika

37

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

"art. no. e24859"

Kód UT WoS článku

000950546300001

EID výsledku v databázi Scopus

2-s2.0-85150656807