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Undertaking the biological sex assessment of human remains: The applicability of minimally-invasive methods for proteomic sex estimation from enamel peptides

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023272%3A_____%2F23%3A10136349" target="_blank" >RIV/00023272:_____/23:10136349 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.sciencedirect.com/science/article/pii/S1296207423002376" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1296207423002376</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.culher.2023.11.021" target="_blank" >10.1016/j.culher.2023.11.021</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Undertaking the biological sex assessment of human remains: The applicability of minimally-invasive methods for proteomic sex estimation from enamel peptides

  • Popis výsledku v původním jazyce

    Being a part of the cultural heritage, skeletal human remains and grave objects are often the only ev- idence of people who lived many years, or even centuries or millennia, ago, and their preservation for future generations is thus of the utmost importance. The first task in analyzing skeletal remains is to build a biological profile of the individual, including in particular a sex estimation. Recently developed proteomic sex analysis, based on the detection of two sex-dependent forms of the amelogenin protein in tooth enamel, could offer a minimally-invasive and reliable approach applicable to both recent and past populations. The aims of the present study are: 1) to validate the proteomic sex estimation approach with a del- icate, minimally-destructive protocol using protein etching in recent and sub-recent identified samples of adult individuals; 2) for the first time, to evaluate the invasiveness of the extraction of amelogenin protein from teeth for proteomic analysis via scanning electron microscope (SEM) and microcomputed tomography (micro-CT); 3) to apply the method to an archaeological sample of unknown adult and juve- nile individuals. An assemblage of 60 teeth (32 males and 28 females) of recent and sub-recent origin was used to validate the approach. A sub-sample of 20 teeth (10 males and 10 females) was used to assess the inva- siveness of the amelogenin extraction procedure. For the application of the method, samples of 15 adult and 32 juvenile teeth, both originating from medieval populations, were used. Proteomic sex estimation achieved 100% accuracy in this sample. An SEM and micro-CT comparison of the dental surfaces before and after chemical treatment showed an approximately 10% loss of enamel and only 2% loss of dentine. The suitability and minimally-invasive character of the protocol for proteomic analysis in biological sex estimation was demonstrated, as was its applicability to archaeological samples.

  • Název v anglickém jazyce

    Undertaking the biological sex assessment of human remains: The applicability of minimally-invasive methods for proteomic sex estimation from enamel peptides

  • Popis výsledku anglicky

    Being a part of the cultural heritage, skeletal human remains and grave objects are often the only ev- idence of people who lived many years, or even centuries or millennia, ago, and their preservation for future generations is thus of the utmost importance. The first task in analyzing skeletal remains is to build a biological profile of the individual, including in particular a sex estimation. Recently developed proteomic sex analysis, based on the detection of two sex-dependent forms of the amelogenin protein in tooth enamel, could offer a minimally-invasive and reliable approach applicable to both recent and past populations. The aims of the present study are: 1) to validate the proteomic sex estimation approach with a del- icate, minimally-destructive protocol using protein etching in recent and sub-recent identified samples of adult individuals; 2) for the first time, to evaluate the invasiveness of the extraction of amelogenin protein from teeth for proteomic analysis via scanning electron microscope (SEM) and microcomputed tomography (micro-CT); 3) to apply the method to an archaeological sample of unknown adult and juve- nile individuals. An assemblage of 60 teeth (32 males and 28 females) of recent and sub-recent origin was used to validate the approach. A sub-sample of 20 teeth (10 males and 10 females) was used to assess the inva- siveness of the amelogenin extraction procedure. For the application of the method, samples of 15 adult and 32 juvenile teeth, both originating from medieval populations, were used. Proteomic sex estimation achieved 100% accuracy in this sample. An SEM and micro-CT comparison of the dental surfaces before and after chemical treatment showed an approximately 10% loss of enamel and only 2% loss of dentine. The suitability and minimally-invasive character of the protocol for proteomic analysis in biological sex estimation was demonstrated, as was its applicability to archaeological samples.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    60102 - Archaeology

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2023

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Journal of Cultural Heritage

  • ISSN

    1296-2074

  • e-ISSN

  • Svazek periodika

    2024

  • Číslo periodika v rámci svazku

    66

  • Stát vydavatele periodika

    FR - Francouzská republika

  • Počet stran výsledku

    11

  • Strana od-do

    204-214

  • Kód UT WoS článku

    001135047200001

  • EID výsledku v databázi Scopus

    2-s2.0-85179078661