Peptide analysis of tooth enamel A sex estimation tool for archaeological, anthropological, or forensic research

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F23%3A00574565" target="_blank" >RIV/67985823:_____/23:00574565 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10465891 RIV/00216275:25310/23:39920291

Výsledek na webu

<a href="https://doi.org/10.1002/jssc.202300183" target="_blank" >https://doi.org/10.1002/jssc.202300183</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202300183" target="_blank" >10.1002/jssc.202300183</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Peptide analysis of tooth enamel A sex estimation tool for archaeological, anthropological, or forensic research

Popis výsledku v původním jazyce

Proteomics has become an attractive method to study human and animal material, biological profile, and origin as an alternative to DNA analysis. It is limited by DNA amplification in ancient samples and its contamination, high cost, and limited preservation of nuclear DNA. Currently, three approaches are available to estimate sex-osteology, genomics, or proteomics, but little is known about the relative reliability of these methods in applied settings. Proteomics provides a new, seemingly simple, and relatively non-expensive way of sex estimation without the risk of contamination. Proteins can be preserved in hard teeth tissue (enamel) for tens of thousands of years. It uses two sexually distinct forms of the protein amelogenin in tooth enamel detectable by liquid chromatography-mass spectrometry, the protein amelogenin Y isoform is present in enamel dental tissue only in males, while amelogenin isoform X can be found in both sexes. From the point of view of archaeological, anthropological, and forensic research and applications, the reduced destruction of the methods used is essential, as well as the minimum requirements for sample size.

Název v anglickém jazyce

Peptide analysis of tooth enamel A sex estimation tool for archaeological, anthropological, or forensic research

Popis výsledku anglicky

Proteomics has become an attractive method to study human and animal material, biological profile, and origin as an alternative to DNA analysis. It is limited by DNA amplification in ancient samples and its contamination, high cost, and limited preservation of nuclear DNA. Currently, three approaches are available to estimate sex-osteology, genomics, or proteomics, but little is known about the relative reliability of these methods in applied settings. Proteomics provides a new, seemingly simple, and relatively non-expensive way of sex estimation without the risk of contamination. Proteins can be preserved in hard teeth tissue (enamel) for tens of thousands of years. It uses two sexually distinct forms of the protein amelogenin in tooth enamel detectable by liquid chromatography-mass spectrometry, the protein amelogenin Y isoform is present in enamel dental tissue only in males, while amelogenin isoform X can be found in both sexes. From the point of view of archaeological, anthropological, and forensic research and applications, the reduced destruction of the methods used is essential, as well as the minimum requirements for sample size.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-03899S" target="_blank" >GA20-03899S: Afinitní kapilární elektrokinetické metody pro selektivní analýzu biopolymerů a metabolitů a pro studium jejich interakcí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

1615-9314

Svazek periodika

46

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

2300183

Kód UT WoS článku

000994804800001

EID výsledku v databázi Scopus

2-s2.0-85160631976