Identifikace nového elementu responzivního na železo v 3'UTR lidské MRCKalfa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F06%3A00006719" target="_blank" >RIV/00023736:_____/06:00006719 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A novel iron responsive element in the 3'UTR of human MRCKalpha

Popis výsledku v původním jazyce

Human untranslated region (UTR) databases were searched to identify novel proteins potentially regulated by an iron responsive element (IRE), and found two candidates ? cell cycle phosphatase Cdc14A variant 1 and myotonic dystrophy kinase-related Cdc42-binding kinase a (MRCKa), both possessing a putative IRE in their 3'UTR. In further experiments, we focused on MRCKa. Biochemical analyses of the MRCKa IRE revealed that it was functional and mediated the response to iron level in the same way as transferrin receptor 1 IREs (TfR) did. Similarly to TfR mRNA, MRCKa mRNA is stabilized, when iron supply is low, while it is destabilized under iron-rich conditions. The expression of MRCKa mRNA was found to be ubiquitous; the highest levels were noted in testes, the lowest in skeletal muscle. The level of MRCKa mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway.

Název v anglickém jazyce

A novel iron responsive element in the 3'UTR of human MRCKalpha

Popis výsledku anglicky

Human untranslated region (UTR) databases were searched to identify novel proteins potentially regulated by an iron responsive element (IRE), and found two candidates ? cell cycle phosphatase Cdc14A variant 1 and myotonic dystrophy kinase-related Cdc42-binding kinase a (MRCKa), both possessing a putative IRE in their 3'UTR. In further experiments, we focused on MRCKa. Biochemical analyses of the MRCKa IRE revealed that it was functional and mediated the response to iron level in the same way as transferrin receptor 1 IREs (TfR) did. Similarly to TfR mRNA, MRCKa mRNA is stabilized, when iron supply is low, while it is destabilized under iron-rich conditions. The expression of MRCKa mRNA was found to be ubiquitous; the highest levels were noted in testes, the lowest in skeletal muscle. The level of MRCKa mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP204%2F04%2FP150" target="_blank" >GP204/04/P150: Identifikace proteinů regulovaných pomocí elementů responzivních na železo (IRE)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical and Biophysical Research Communications.

ISSN

0006-291X

e-ISSN

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Svazek periodika

341

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

158-166

Kód UT WoS článku

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EID výsledku v databázi Scopus

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