Fibrin clot formation under oxidative stress conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F21%3A00013249" target="_blank" >RIV/00023736:_____/21:00013249 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61383082:_____/21:00001054 RIV/00216208:11110/21:10429390

Výsledek na webu

<a href="https://doi.org/10.3390/antiox10060923" target="_blank" >https://doi.org/10.3390/antiox10060923</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/antiox10060923" target="_blank" >10.3390/antiox10060923</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fibrin clot formation under oxidative stress conditions

Popis výsledku v původním jazyce

The aim was to examine the effects of oxidative stress conditions on fibrin clot formation in arterial atherothrombotic disorders. We studied the changes in in vitro fibrin network formation in three groups of patients—with acute coronary syndrome (ACS), with significant carotid artery stenosis (SCAS), and with acute ischemic stroke (AIS), as well as a control group. The level of oxidative stress marker malondialdehyde measured by LC-MS/MS was higher in SCAS and AIS patients compared with controls. Turbidic methods revealed a higher final optical density and a prolonged lysis time in the clots of these patients. Electron microscopy was used to visualize changes in the in vitro-formed fibrin network. Fibers from patients with AIS were significantly thicker in comparison with control and ACS fibers. The number of fibrin fibers in patients with AIS was significantly lower in comparison with ACS and control groups. Thus, oxidative stress-medi-ated changes in fibrin clot formation, structure and dissolution may affect the effectiveness of thrombolytic therapy.

Název v anglickém jazyce

Fibrin clot formation under oxidative stress conditions

Popis výsledku anglicky

The aim was to examine the effects of oxidative stress conditions on fibrin clot formation in arterial atherothrombotic disorders. We studied the changes in in vitro fibrin network formation in three groups of patients—with acute coronary syndrome (ACS), with significant carotid artery stenosis (SCAS), and with acute ischemic stroke (AIS), as well as a control group. The level of oxidative stress marker malondialdehyde measured by LC-MS/MS was higher in SCAS and AIS patients compared with controls. Turbidic methods revealed a higher final optical density and a prolonged lysis time in the clots of these patients. Electron microscopy was used to visualize changes in the in vitro-formed fibrin network. Fibers from patients with AIS were significantly thicker in comparison with control and ACS fibers. The number of fibrin fibers in patients with AIS was significantly lower in comparison with ACS and control groups. Thus, oxidative stress-medi-ated changes in fibrin clot formation, structure and dissolution may affect the effectiveness of thrombolytic therapy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30205 - Hematology

Projekt

<a href="/cs/project/NV18-08-00149" target="_blank" >NV18-08-00149: Kritické zhodnocení lipidomu u pacientů s akutním koronárním syndromem a cévní mozkovou příhodou ve vztahu ke stupni oxidačního stresu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Antioxidants

ISSN

2076-3921

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

"art. no. 923"

Kód UT WoS článku

000665570200001

EID výsledku v databázi Scopus

2-s2.0-85107281202