Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutant

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F25%3A00013793" target="_blank" >RIV/00023736:_____/25:00013793 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/25:10498712 RIV/00216208:11310/25:10498712

Výsledek na webu

<a href="https://doi.org/10.1371/journal.pone.0322096" target="_blank" >https://doi.org/10.1371/journal.pone.0322096</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0322096" target="_blank" >10.1371/journal.pone.0322096</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutant

Popis výsledku v původním jazyce

Tumor suppressor p53 is a key player in the cell response to DNA damage that suffers by frequent inactivating aberrations. Some of them disturb p53 oligomerization and influence cell decision between proliferation, growth arrest and apoptosis. Active p53 resides mostly in the nucleus, degradation occurs in the cytoplasm. Acute myeloid leukemia (AML)-related mutation of NPM (NPMmut) induces massive mislocalization of p53 to the cytoplasm, which might be related to leukemia initiation. Since both proteins interact and execute their function as oligomers, we investigated the role of perturbed p53 oligomerization in the p53 mislocalization process in live cells by FLIM (fluorescence lifetime imaging microscopy), fluorescence anisotropy imaging (FAIM), fluorescence cross-correlation spectroscopy (FCCS) and immunochemical methods.

Název v anglickém jazyce

Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutant

Popis výsledku anglicky

Tumor suppressor p53 is a key player in the cell response to DNA damage that suffers by frequent inactivating aberrations. Some of them disturb p53 oligomerization and influence cell decision between proliferation, growth arrest and apoptosis. Active p53 resides mostly in the nucleus, degradation occurs in the cytoplasm. Acute myeloid leukemia (AML)-related mutation of NPM (NPMmut) induces massive mislocalization of p53 to the cytoplasm, which might be related to leukemia initiation. Since both proteins interact and execute their function as oligomers, we investigated the role of perturbed p53 oligomerization in the p53 mislocalization process in live cells by FLIM (fluorescence lifetime imaging microscopy), fluorescence anisotropy imaging (FAIM), fluorescence cross-correlation spectroscopy (FCCS) and immunochemical methods.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30205 - Hematology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS one

ISSN

1932-6203

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

26

Strana od-do

"art.no. e0322096"

Kód UT WoS článku

001488722300037

EID výsledku v databázi Scopus

2-s2.0-105004781315