Protein tau-mediated effects on rat hippocampal choline transporters CHT1 and tau-amyloid beta interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F13%3A43914467" target="_blank" >RIV/00023752:_____/13:43914467 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs11064-013-1101-5" target="_blank" >http://link.springer.com/article/10.1007%2Fs11064-013-1101-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11064-013-1101-5" target="_blank" >10.1007/s11064-013-1101-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protein tau-mediated effects on rat hippocampal choline transporters CHT1 and tau-amyloid beta interactions

Popis výsledku v původním jazyce

Intracellular tau protein, when released extracellularly upon neuron degeneration, could evoke direct toxic effects on the cholinergic neurotransmitter system through muscarinic receptors and thus contribute to the pathogenesis of Alzheimer's disease. Weevaluated the in vitro effects of six naturally occurring monomeric tau isoforms on rat hippocampal synaptosomal choline transporters CHT1. Some tau isoforms at nM concentrations inhibited choline transport in a dose- and time-dependent saturable manner(352 = 441 > 410 = 383 > 381 = 412) and effects were associated with changes in the Michaelis constant rather than in maximal velocity. Moreover, the actions of tau 352/441 were not influenced by previous depolarisation of synaptosomes or by previous depletion of membrane cholesterol. Results obtained on CHT1 transporters from cholesterol-depleted synaptosomes supported interactions between amyloid beta 1-40 and tau 352. It seems that protein tau, similar to amyloid beta, can contribute

Název v anglickém jazyce

Protein tau-mediated effects on rat hippocampal choline transporters CHT1 and tau-amyloid beta interactions

Popis výsledku anglicky

Intracellular tau protein, when released extracellularly upon neuron degeneration, could evoke direct toxic effects on the cholinergic neurotransmitter system through muscarinic receptors and thus contribute to the pathogenesis of Alzheimer's disease. Weevaluated the in vitro effects of six naturally occurring monomeric tau isoforms on rat hippocampal synaptosomal choline transporters CHT1. Some tau isoforms at nM concentrations inhibited choline transport in a dose- and time-dependent saturable manner(352 = 441 > 410 = 383 > 381 = 412) and effects were associated with changes in the Michaelis constant rather than in maximal velocity. Moreover, the actions of tau 352/441 were not influenced by previous depolarisation of synaptosomes or by previous depletion of membrane cholesterol. Results obtained on CHT1 transporters from cholesterol-depleted synaptosomes supported interactions between amyloid beta 1-40 and tau 352. It seems that protein tau, similar to amyloid beta, can contribute

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neurochemical Research

ISSN

0364-3190

e-ISSN

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Svazek periodika

38

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1949-1959

Kód UT WoS článku

000322722700021

EID výsledku v databázi Scopus

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