Protein ?-mediated effects on rat hippocampal choline transporters CHT1 and ?-amyloid ? interactions
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F13%3A00396521" target="_blank" >RIV/67985882:_____/13:00396521 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s11064-013-1101-5" target="_blank" >http://dx.doi.org/10.1007/s11064-013-1101-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11064-013-1101-5" target="_blank" >10.1007/s11064-013-1101-5</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Protein ?-mediated effects on rat hippocampal choline transporters CHT1 and ?-amyloid ? interactions
Popis výsledku v původním jazyce
It is suggested that intracellular tau protein (?), when released extracellularly upon neuron degeneration, could evoke direct toxic effects on the cholinergic neurotransmitter system through muscarinic receptors and thus contribute to the pathogenesis of Alzheimer's disease. In this study, we evaluated the in vitro effects of six naturally occurring monomeric ? isoforms on rat hippocampal synaptosomal choline transporters CHT1 (large transmembrane proteins associated with high-affinity choline transport and vulnerable to actions of amyloid ? peptides (A?) applied in vitro or in vivo). Some ? isoforms at nM concentrations inhibited choline transport in a dose- and time-dependent saturable manner (352 = 441 > 410 = 383 > 381 = 412) and effects were associated with changes in the Michaelis constant rather than in maximal velocity. Moreover, the actions of ? 352/441 were not influenced by previous depolarisation of synaptosomes or by previous depletion of membrane cholesterol. Specific bi
Název v anglickém jazyce
Protein ?-mediated effects on rat hippocampal choline transporters CHT1 and ?-amyloid ? interactions
Popis výsledku anglicky
It is suggested that intracellular tau protein (?), when released extracellularly upon neuron degeneration, could evoke direct toxic effects on the cholinergic neurotransmitter system through muscarinic receptors and thus contribute to the pathogenesis of Alzheimer's disease. In this study, we evaluated the in vitro effects of six naturally occurring monomeric ? isoforms on rat hippocampal synaptosomal choline transporters CHT1 (large transmembrane proteins associated with high-affinity choline transport and vulnerable to actions of amyloid ? peptides (A?) applied in vitro or in vivo). Some ? isoforms at nM concentrations inhibited choline transport in a dose- and time-dependent saturable manner (352 = 441 > 410 = 383 > 381 = 412) and effects were associated with changes in the Michaelis constant rather than in maximal velocity. Moreover, the actions of ? 352/441 were not influenced by previous depolarisation of synaptosomes or by previous depletion of membrane cholesterol. Specific bi
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
JA - Elektronika a optoelektronika, elektrotechnika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Neurochemical Research
ISSN
0364-3190
e-ISSN
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Svazek periodika
38
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
1949-1959
Kód UT WoS článku
000322722700021
EID výsledku v databázi Scopus
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