Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F20%3A43920010" target="_blank" >RIV/00023752:_____/20:43920010 - isvavai.cz</a>

Výsledek na webu

<a href="https://iopscience.iop.org/article/10.1088/1748-605X/ab52db" target="_blank" >https://iopscience.iop.org/article/10.1088/1748-605X/ab52db</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1748-605X/ab52db" target="_blank" >10.1088/1748-605X/ab52db</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies

Popis výsledku v původním jazyce

Decellularized human pericardium is under study as an allogenic material for cardiovascular application purposes. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined via a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined via multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin were evaluated by means of an MTS assay. The viability of the cells measured via their metabolic activity decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a way by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.

Název v anglickém jazyce

Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies

Popis výsledku anglicky

Decellularized human pericardium is under study as an allogenic material for cardiovascular application purposes. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined via a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined via multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin were evaluated by means of an MTS assay. The viability of the cells measured via their metabolic activity decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a way by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Materials

ISSN

1748-605X

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

"Article number 015008"

Kód UT WoS článku

000563778900006

EID výsledku v databázi Scopus

2-s2.0-85076327091