Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00532330" target="_blank" >RIV/61389013:_____/20:00532330 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985823:_____/20:00532330 RIV/00216208:11510/20:10401166 RIV/68407700:21460/20:00342548 RIV/00023001:_____/20:00079475
Výsledek na webu
<a href="https://doi.org/10.1088/1748-605X/ab52db" target="_blank" >https://doi.org/10.1088/1748-605X/ab52db</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1748-605X/ab52db" target="_blank" >10.1088/1748-605X/ab52db</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies
Popis výsledku v původním jazyce
Decellularized human pericardium is under study as an allogenic material for cardiovascular applications. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined with a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined by multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin was evaluated by means of an MTS assay. The viability of the cells, measured by their metabolic activity, decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a method by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.
Název v anglickém jazyce
Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies
Popis výsledku anglicky
Decellularized human pericardium is under study as an allogenic material for cardiovascular applications. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined with a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined by multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin was evaluated by means of an MTS assay. The viability of the cells, measured by their metabolic activity, decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a method by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
<a href="/cs/project/NV15-29153A" target="_blank" >NV15-29153A: Vývoj aortální chlopně na bázi perikardu pomocí primárních a kmenových buněk a mechanického zatěžování v bioreaktoru</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biomedical Materials
ISSN
1748-6041
e-ISSN
—
Svazek periodika
15
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
015008
Kód UT WoS článku
000563778900006
EID výsledku v databázi Scopus
2-s2.0-85076327091