A Method for Determination of One Hundred Endogenous Steroids in Human Serum by Gas Chromatography-Tandem Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F19%3AN0000027" target="_blank" >RIV/00023761:_____/19:N0000027 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10397385 RIV/00064165:_____/19:10397385

Výsledek na webu

<a href="http://www.biomed.cas.cz/physiolres/pdf/2019/68_179.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/2019/68_179.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934124" target="_blank" >10.33549/physiolres.934124</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Method for Determination of One Hundred Endogenous Steroids in Human Serum by Gas Chromatography-Tandem Mass Spectrometry

Popis výsledku v původním jazyce

Steroid profiling helps various pathologies to be rapidly diagnosed. Results from analyses investigating steroidogenic pathways may be used as a tool for uncovering pathology causations and proposals of new therapeutic approaches. The purpose of this study was to address still underutilized application of the advanced GC-MS/MS platform for the multicomponent quantification of endogenous steroids. We developed and validated a GC-MS/MS method for the quantification of 58 unconjugated steroids and 42 polar conjugates of steroids (after hydrolysis) in human blood. The present method was validated not only for blood of men and non-pregnant women but also for blood of pregnant women and for mixed umbilical cord blood. The spectrum of analytes includes common hormones operating via nuclear receptors as well as other bioactive substances like immunomodulatory and neuroactive steroids. Our present results are comparable with those from our previously published GC-MS method as well as the results of others. The present method was extended for corticoids and 17 alpha-hydroxylated 5 alpha/beta-reduced pregnanes, which are useful for the investigation of alternative "backdoor" pathway. When comparing the analytical characteristics of the present and previous method, the first exhibit by far higher selectivity, and generally generally higher sensitivity and better precision particularly for 17 alpha-hydroxysteroids.

Název v anglickém jazyce

A Method for Determination of One Hundred Endogenous Steroids in Human Serum by Gas Chromatography-Tandem Mass Spectrometry

Popis výsledku anglicky

Steroid profiling helps various pathologies to be rapidly diagnosed. Results from analyses investigating steroidogenic pathways may be used as a tool for uncovering pathology causations and proposals of new therapeutic approaches. The purpose of this study was to address still underutilized application of the advanced GC-MS/MS platform for the multicomponent quantification of endogenous steroids. We developed and validated a GC-MS/MS method for the quantification of 58 unconjugated steroids and 42 polar conjugates of steroids (after hydrolysis) in human blood. The present method was validated not only for blood of men and non-pregnant women but also for blood of pregnant women and for mixed umbilical cord blood. The spectrum of analytes includes common hormones operating via nuclear receptors as well as other bioactive substances like immunomodulatory and neuroactive steroids. Our present results are comparable with those from our previously published GC-MS method as well as the results of others. The present method was extended for corticoids and 17 alpha-hydroxylated 5 alpha/beta-reduced pregnanes, which are useful for the investigation of alternative "backdoor" pathway. When comparing the analytical characteristics of the present and previous method, the first exhibit by far higher selectivity, and generally generally higher sensitivity and better precision particularly for 17 alpha-hydroxysteroids.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/NV17-30528A" target="_blank" >NV17-30528A: Predikce gestačního diabetu na základě metabolizmu steroidů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PHYSIOLOGICAL RESEARCH

ISSN

0862-8408

e-ISSN

1802-9973

Svazek periodika

68

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

29

Strana od-do

179-207

Kód UT WoS článku

000465948800006

EID výsledku v databázi Scopus

2-s2.0-85065492017