An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F22%3AN0000010" target="_blank" >RIV/00023761:_____/22:N0000010 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/22:43925435

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1570023222001982" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1570023222001982</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2022.123294" target="_blank" >10.1016/j.jchromb.2022.123294</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma

Popis výsledku v původním jazyce

A development of robust and rapid method with simple sample preparation for the analysis of steroids of C18-, C19-, C21- families is of interest of many research groups. Here we present a novel LC-MS/MS method for the simultaneous quantification of 32 steroid hormones in human plasma. Twenty-two of them were analyzed directly without the need for derivatization, while ten were derivatized with 2-fluoro-1-methylpyridinium p-toluenesulfonate. The steroids were separated on a C18 column with a gradient elution consisting of methanol and water with the addition of 0.1% formic acid. The mass spectrometer was operated in positive ESI mode. Validation demonstrated that the method was applicable for the quantitative analysis of two C18- steroids (estrone, estradiol), nineteen C19- steroids (testosterone, epitestosterone, dihydrotestosterone, 11-ketodihydrotestosterone, 11 beta-hydroxyandrostenedione, 11 beta-hydroxytestosterone, 11-ketotestosterone, dehydroepiandrosterone, 7 alpha-hydroxydehydroepiandrosterone, 7 beta hydroxydehydroepiandrosterone, 7-ketodehydroepiandrosterone, androsterone, epiandrosterone, androstenedione, androstenediol, 5 alpha-androstane-3 alpha,17 beta-diol, 5 alpha-androstane-3 beta,17 beta-diol, 5 beta-androstane-3 alpha,17 beta-diol, 5 beta-androstane-3 beta,17 beta-diol), and eleven C21- steroids (cortisol, 21-deoxycortisol, 11-deoxycortisol, cortisone, corticosterone, 11-deoxycorticosterone, pregnenolone, 17 -hydroxypregnenolone, progesterone, 17-hydroxyprogesterone, 5 alpha-dihydroprogesterone). The lower limits of quantification are appropriate for analyses in both physiological and various pathophysiological conditions. The accuracy, intra-and inter-day precision values as well as stability tests were in accordance with FDA Guidelines. The method will be a useful tool in investigating the mechanisms of steroid-related diseases and will serve as a steppingstone for the development of other methods for steroid analyses in various biological matrices such as prostate tissue, cerebrospinal fluid, urine, seminal fluid, and saliva.

Název v anglickém jazyce

An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma

Popis výsledku anglicky

A development of robust and rapid method with simple sample preparation for the analysis of steroids of C18-, C19-, C21- families is of interest of many research groups. Here we present a novel LC-MS/MS method for the simultaneous quantification of 32 steroid hormones in human plasma. Twenty-two of them were analyzed directly without the need for derivatization, while ten were derivatized with 2-fluoro-1-methylpyridinium p-toluenesulfonate. The steroids were separated on a C18 column with a gradient elution consisting of methanol and water with the addition of 0.1% formic acid. The mass spectrometer was operated in positive ESI mode. Validation demonstrated that the method was applicable for the quantitative analysis of two C18- steroids (estrone, estradiol), nineteen C19- steroids (testosterone, epitestosterone, dihydrotestosterone, 11-ketodihydrotestosterone, 11 beta-hydroxyandrostenedione, 11 beta-hydroxytestosterone, 11-ketotestosterone, dehydroepiandrosterone, 7 alpha-hydroxydehydroepiandrosterone, 7 beta hydroxydehydroepiandrosterone, 7-ketodehydroepiandrosterone, androsterone, epiandrosterone, androstenedione, androstenediol, 5 alpha-androstane-3 alpha,17 beta-diol, 5 alpha-androstane-3 beta,17 beta-diol, 5 beta-androstane-3 alpha,17 beta-diol, 5 beta-androstane-3 beta,17 beta-diol), and eleven C21- steroids (cortisol, 21-deoxycortisol, 11-deoxycortisol, cortisone, corticosterone, 11-deoxycorticosterone, pregnenolone, 17 -hydroxypregnenolone, progesterone, 17-hydroxyprogesterone, 5 alpha-dihydroprogesterone). The lower limits of quantification are appropriate for analyses in both physiological and various pathophysiological conditions. The accuracy, intra-and inter-day precision values as well as stability tests were in accordance with FDA Guidelines. The method will be a useful tool in investigating the mechanisms of steroid-related diseases and will serve as a steppingstone for the development of other methods for steroid analyses in various biological matrices such as prostate tissue, cerebrospinal fluid, urine, seminal fluid, and saliva.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU21J-01-00040" target="_blank" >NU21J-01-00040: Intraprostatické steroidy: úloha alternativních biosyntetických drah a endokrinních disruptorů v patogenezi karcinomu prostaty</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

ISSN

1570-0232

e-ISSN

1873-376X

Svazek periodika

1201

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

Article Number123294

Kód UT WoS článku

000806812600002

EID výsledku v databázi Scopus

2-s2.0-85130593000