LC-MS/MS techniques for the analysis of steroid panel in human cerebrospinal fluid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F25%3AN0000014" target="_blank" >RIV/00023761:_____/25:N0000014 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61383082:_____/25:00001576 RIV/00216208:11110/25:10504535 RIV/00216208:11130/25:10504535 RIV/00064203:_____/25:10504535

Výsledek na webu

<a href="https://doi.org/10.1016/j.neuint.2025.106080" target="_blank" >https://doi.org/10.1016/j.neuint.2025.106080</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.neuint.2025.106080" target="_blank" >10.1016/j.neuint.2025.106080</a>

Jazyk výsledku

angličtina

Název v původním jazyce

LC-MS/MS techniques for the analysis of steroid panel in human cerebrospinal fluid

Popis výsledku v původním jazyce

The metabolic processes within the brain are reflected in the cerebrospinal fluid (CSF). It is in close contact with the nervous system, which is both target and source of multiple steroids. The aim of our study was to develop and validate robust, sensitive LC-MS/MS methods with and without derivatization step for the analysis of unconjugated steroids from all major steroid classes in CSF. The validation of the method without derivatization was performed for ten C19- steroids (dehydroepiandrosterone (DHEA), 7a-hydroxyDHEA, 7(3-hydroxyDHEA, 7ketoDHEA, testosterone, epitestosterone, dihydrotestosterone, 11-hydroxytestosterone, 11-ketotestosterone and androstenedione), ten C21- steroids (cortisol, 11-deoxycortisol, 21-deoxycortisol, cortisone, corticosterone, 11deoxycorticosterone, pregnenolone, progesterone, 17-hydroxyprogesterone, aldosterone) and three C18- steroids (estrone, estradiol, estriol). The method with derivatization is validated for determination of eleven C19steroids (testosterone, 11-ketodihydrotestosterone, 11-hydroxytestosterone, DHEA, 7a-hydroxyDHEA, 7(3hydroxyDHEA, 7-ketoDHEA, androstenedione, androsterone, epiandrosterone, 7(3-hydroxyepiandrosterone) and six C21- steroids (cortisol, cortisone, corticosterone, pregnenolone, 17-hydroxypregnenolone, progesterone) in CSF. The method without derivatization is applicable for the determination of the majority of steroids in CSF, except for pregnenolone, 17-hydroxypregnenolone and DHEA, for which the derivatization method provides better sensitivity. When analyzing CSF samples of normal pressure hydrocephalus (NPH) patients, 11-ketodihydrotestosterone, epitestosterone, androsterone, epiandrosterone, 7(3-hydroxyepiandrosterone, 7-ketoDHEA and 21-deoxycortisol were found to be below the LLOQ, suggesting that their presence is very limited. 17-hydroxypregnenolone, and 11-deoxycortisol were quantified for the first time, their CSF levels in NPH subjects are presented. We also observed significantly increased CSF levels of testosterone and 17-hydroxyprogesterone in men compared to women, both with NPH.

Název v anglickém jazyce

LC-MS/MS techniques for the analysis of steroid panel in human cerebrospinal fluid

Popis výsledku anglicky

The metabolic processes within the brain are reflected in the cerebrospinal fluid (CSF). It is in close contact with the nervous system, which is both target and source of multiple steroids. The aim of our study was to develop and validate robust, sensitive LC-MS/MS methods with and without derivatization step for the analysis of unconjugated steroids from all major steroid classes in CSF. The validation of the method without derivatization was performed for ten C19- steroids (dehydroepiandrosterone (DHEA), 7a-hydroxyDHEA, 7(3-hydroxyDHEA, 7ketoDHEA, testosterone, epitestosterone, dihydrotestosterone, 11-hydroxytestosterone, 11-ketotestosterone and androstenedione), ten C21- steroids (cortisol, 11-deoxycortisol, 21-deoxycortisol, cortisone, corticosterone, 11deoxycorticosterone, pregnenolone, progesterone, 17-hydroxyprogesterone, aldosterone) and three C18- steroids (estrone, estradiol, estriol). The method with derivatization is validated for determination of eleven C19steroids (testosterone, 11-ketodihydrotestosterone, 11-hydroxytestosterone, DHEA, 7a-hydroxyDHEA, 7(3hydroxyDHEA, 7-ketoDHEA, androstenedione, androsterone, epiandrosterone, 7(3-hydroxyepiandrosterone) and six C21- steroids (cortisol, cortisone, corticosterone, pregnenolone, 17-hydroxypregnenolone, progesterone) in CSF. The method without derivatization is applicable for the determination of the majority of steroids in CSF, except for pregnenolone, 17-hydroxypregnenolone and DHEA, for which the derivatization method provides better sensitivity. When analyzing CSF samples of normal pressure hydrocephalus (NPH) patients, 11-ketodihydrotestosterone, epitestosterone, androsterone, epiandrosterone, 7(3-hydroxyepiandrosterone, 7-ketoDHEA and 21-deoxycortisol were found to be below the LLOQ, suggesting that their presence is very limited. 17-hydroxypregnenolone, and 11-deoxycortisol were quantified for the first time, their CSF levels in NPH subjects are presented. We also observed significantly increased CSF levels of testosterone and 17-hydroxyprogesterone in men compared to women, both with NPH.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/NU23-04-00551" target="_blank" >NU23-04-00551: Komplexní multidoménová diagnostická baterie pro NPH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neurochemistry International

ISSN

0197-0186

e-ISSN

1872-9754

Svazek periodika

191

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

106080

Kód UT WoS článku

001609422400001

EID výsledku v databázi Scopus

2-s2.0-105022182974