Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023817%3A_____%2F03%3A%230000001" target="_blank" >RIV/00023817:_____/03:#0000001 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.200301441" target="_blank" >http://dx.doi.org/10.1002/jssc.200301441</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.200301441" target="_blank" >10.1002/jssc.200301441</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Popis výsledku v původním jazyce

A micellar electrokinetic chromatographic and a fast reversed-phase liquid chromatographic method have been developed for determination of the purity of phenoxymethylpenicillin. The optimized running buffer composition was 40 mM phosphate?borate?125 mM SDS?3.5% (v/v) methanol. The HPLC method employed a monolithic silica C18 column and a mobile phase composed of phosphate buffer, pH 3.5, and ACN, the flow-rate being 3.5 mL/min. Both methods were successfully validated. Linearity, intermediate precision,limits of quantitation, accuracy, and a good correlation of the HPLC and MEKC results were demonstrated. Both methods proved to be fast and reliable and sufficiently sensitive. A combination of the two methods can be very useful in impurity profiling.

Název v anglickém jazyce

Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Popis výsledku anglicky

A micellar electrokinetic chromatographic and a fast reversed-phase liquid chromatographic method have been developed for determination of the purity of phenoxymethylpenicillin. The optimized running buffer composition was 40 mM phosphate?borate?125 mM SDS?3.5% (v/v) methanol. The HPLC method employed a monolithic silica C18 column and a mobile phase composed of phosphate buffer, pH 3.5, and ACN, the flow-rate being 3.5 mL/min. Both methods were successfully validated. Linearity, intermediate precision,limits of quantitation, accuracy, and a good correlation of the HPLC and MEKC results were demonstrated. Both methods proved to be fast and reliable and sufficiently sensitive. A combination of the two methods can be very useful in impurity profiling.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/NL5965" target="_blank" >NL5965: Kontrola obsahu příbuzných látek amoxicilinu,ampicilinu,fenoxymethylpenicilinu v léčivých přípravcích za použití metod HPLC a CE</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of separation science

ISSN

1615-9314

e-ISSN

—

Svazek periodika

26

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

701-708

Kód UT WoS článku

000183660100010

EID výsledku v databázi Scopus

—