Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F03%3A33" target="_blank" >RIV/00027006:_____/03:33 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population

Popis výsledku v původním jazyce

The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection ofCpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population.

Název v anglickém jazyce

Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population

Popis výsledku anglicky

The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection ofCpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Virologica

ISSN

0001-723X

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

5

Strana od-do

153-157

Kód UT WoS článku

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EID výsledku v databázi Scopus

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